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首页> 外文期刊>Applied Microbiology >Diet Complexity and Estrogen Receptor β Status Affect the Composition of the Murine Intestinal Microbiota
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Diet Complexity and Estrogen Receptor β Status Affect the Composition of the Murine Intestinal Microbiota

机译：饮食复杂性和雌激素受体β状态影响小鼠肠道菌群的组成

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Intestinal microbial dysbiosis contributes to the dysmetabolism of luminal factors, including steroid hormones (sterones) that affect the development of chronic gastrointestinal inflammation and the incidence of sterone-responsive cancers of the breast, prostate, and colon. Little is known, however, about the role of specific host sterone nucleoreceptors, including estrogen receptor β (ERβ), in microbiota maintenance. Herein, we test the hypothesis that ERβ status affects microbiota composition and determine if such compositionally distinct microbiota respond differently to changes in diet complexity that favor Proteobacteria enrichment. To this end, conventionally raised female ERβ~(+/+) and ERβ~(?/?) C57BL/6J mice (mean age of 27 weeks) were initially reared on 8604, a complex diet containing estrogenic isoflavones, and then fed AIN-76, an isoflavone-free semisynthetic diet, for 2 weeks. 16S rRNA gene surveys revealed that the fecal microbiota of 8604-fed mice and AIN-76-fed mice differed, as expected. The relative diversity of Proteobacteria , especially the Alphaproteobacteria and Gammaproteobacteria , increased significantly following the transition to AIN-76. Distinct patterns for beneficial Lactobacillales were exclusive to and highly abundant among 8604-fed mice, whereas several Proteobacteria were exclusive to AIN-76-fed mice. Interestingly, representative orders of the phyla Proteobacteria , Bacteroidetes , and Firmicutes , including the Lactobacillales , also differed as a function of murine ERβ status. Overall, these interactions suggest that sterone nucleoreceptor status and diet complexity may play important roles in microbiota maintenance. Furthermore, we envision that this model for gastrointestinal dysbiosis may be used to identify novel probiotics, prebiotics, nutritional strategies, and pharmaceuticals for the prevention and resolution of Proteobacteria -rich dysbiosis.
机译：肠道微生物代谢异常是导致管腔因子代谢失调的原因，其中包括类固醇激素（类固醇），这些激素会影响慢性胃肠道炎症的发展以及对乳腺癌，前列腺癌和结肠癌的甾体反应性癌症的发生率。然而，关于特定宿主固醇核受体（包括雌激素受体β（ERβ））在微生物群维持中的作用知之甚少。在本文中，我们检验了ERβ状态会影响微生物群组成的假设，并确定这种组成不同的微生物群是否对有利于变形杆菌富集的饮食复杂性变化做出不同反应。为此，首先将常规饲养的雌性雌性ERβ〜（+ / +）和ERβ〜（α/β）C57BL / 6J小鼠（平均年龄27周）饲养在8604（一种含有雌激素异黄酮的复杂饮食）中，然后喂食AIN -76，无异黄酮半合成饮食，持续2周。 16S rRNA基因调查显示，按预期，8604喂养的小鼠和AIN-76喂养的小鼠的粪便菌群有所不同。过渡到AIN-76后，变形杆菌的相对多样性，尤其是Alphaproteobacteria和Gammaproteobacteria明显增加。有益乳杆菌的独特模式是8604喂养的小鼠所独有的，并且高度丰富，而几种变形杆菌则是AIN-76喂养的小鼠所独有的。有趣的是，门菌的细菌，拟杆菌和硬菌（包括乳杆菌）的代表性顺序也因鼠ERβ状态而异。总体而言，这些相互作用表明，固醇核受体的状态和饮食的复杂性可能在微生物群的维持中起重要作用。此外，我们预想这种胃肠道营养不良的模型可用于识别新型益生菌，益生元，营养策略和药物，以预防和解决富含变形杆菌的营养不良。

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