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首页> 外文期刊>Applied Microbiology >prfA-Like Transcription Factor Gene lmo0753 Contributes to l-Rhamnose Utilization in Listeria monocytogenes Strains Associated with Human Food-Borne Infections
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prfA-Like Transcription Factor Gene lmo0753 Contributes to l-Rhamnose Utilization in Listeria monocytogenes Strains Associated with Human Food-Borne Infections

机译：prfA样转录因子基因lmo0753有助于与人食源性感染相关的单核细胞增生李斯特菌菌株的l-鼠李糖利用

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Listeria monocytogenes is a food-borne bacterial pathogen and the causative agent of human and animal listeriosis. Among the three major genetic lineages of L. monocytogenes (i.e., LI, LII, and LIII), LI and LII are predominantly associated with food-borne listeriosis outbreaks, whereas LIII is rarely implicated in human infections. In a previous study, we identified a Crp/Fnr family transcription factor gene, lmo0753 , that was highly specific to outbreak-associated LI and LII but absent from LIII. Lmo0753 shares two conserved functional domains, including a DNA binding domain, with the well-characterized master virulence regulator PrfA in L. monocytogenes . In this study, we constructed lmo0753 deletion and complementation mutants in two fully sequenced L. monocytogenes LII strains, 10403S and EGDe, and compared the flagellar motility, phospholipase C production, hemolysis, and intracellular growth of the mutants and their respective wild types. Our results suggested that lmo0753 plays a role in hemolytic activity in both EGDe and 10403S. More interestingly, we found that deletion of lmo0753 led to the loss of l-rhamnose utilization in EGDe, but not in 10403S. RNA-seq analysis of EGDe Δ 0753 incubated in phenol red medium containing l-rhamnose as the sole carbon source revealed that 126 (4.5%) and 546 (19.5%) out of 2,798 genes in the EGDe genome were up- and downregulated more than 2-fold, respectively, compared to the wild-type strain. Genes related to biotin biosynthesis, general stress response, and rhamnose metabolism were shown to be differentially regulated. Findings from this study collectively suggested varied functional roles of lmo0753 in different LII L. monocytogenes strain backgrounds associated with human listeriosis outbreaks.
机译：单核细胞增生李斯特菌是食源性细菌病原体，是人类和动物李斯特菌病的病原体。在单核细胞增生李斯特氏菌的三个主要遗传谱系中（即LI，LII和LIII），LI和LII主要与食源性李斯特菌病暴发有关，而LIII很少涉及人类感染。在先前的研究中，我们鉴定了Crp / Fnr家族转录因子基因lmo0753，该基因对与爆发相关的LI和LII具有高度特异性，但在LIII中却没有。 Lmo0753与单核细胞增生李斯特菌中特征明确的主毒力调节剂PrfA共享两个保守的功能域，包括一个DNA结合域。在这项研究中，我们在两个完全测序的单核细胞增生李斯特氏菌LII菌株10403S和EGDe中构建了lmo0753缺失和互补突变体，并比较了该鞭毛的运动性，磷脂酶C的产生，溶血作用以及这些突变体及其各自野生型的细胞内生长。我们的结果表明，lmo0753在EGDe和10403S中均在溶血活性中起作用。更有趣的是，我们发现删除lmo0753会导致EGDe中l-鼠李糖的利用率下降，而在10403S中则不会。在含有l-鼠李糖作为唯一碳源的酚红培养基中孵育的EGDeΔ0753的RNA-seq分析显示，在EGDe基因组的2798个基因中，分别有126个（4.5％）和546个（546.19.5％）基因上调和下调的程度超过分别是野生型菌株的2倍。与生物素生物合成，一般应激反应和鼠李糖代谢有关的基因显示受到差异调节。这项研究的发现共同提示了lmo0753在与人类李斯特菌病暴发相关的不同LII单核细胞增生李斯特菌菌株背景中的不同功能作用。

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