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首页> 外文期刊>Applied Microbiology >A Single Mutation in the Gene Responsible for the Mucoid Phenotype of Bifidobacterium animalis subsp. lactis Confers Surface and Functional Characteristics
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A Single Mutation in the Gene Responsible for the Mucoid Phenotype of Bifidobacterium animalis subsp. lactis Confers Surface and Functional Characteristics

机译：负责动物双歧杆菌亚种粘液表型的基因的单一突变。乳酸赋予表面和功能特性

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摘要

Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. We have sequenced the genomes of two isogenic strains of Bifidobacterium animalis subsp. lactis that differ in their EPS-producing phenotype. The original strain displays a nonmucoid appearance, and the mutant derived thereof has acquired a mucoid phenotype. The sequence analysis of their genomes revealed a nonsynonymous mutation in the gene Balat_1410, putatively involved in the elongation of the EPS chain. By comparing a strain from which this gene had been deleted with strains containing the wild-type and mutated genes, we were able to show that each strain displays different cell surface characteristics. The mucoid EPS synthesized by the strain harboring the mutation in Balat_1410 provided higher resistance to gastrointestinal conditions and increased the capability for adhesion to human enterocytes. In addition, the cytokine profiles of human peripheral blood mononuclear cells and ex vivo colon tissues suggest that the mucoid strain could have higher anti-inflammatory activity. Our findings provide relevant data on the function of Balat_1410 and reveal that the mucoid phenotype is able to alter some of the most relevant functional properties of the cells.
机译：外多糖（EPS）是由多种细菌合成的细胞外碳水化合物聚合物。它们的生理功能已被广泛研究，但它们的许多作用尚未阐明。我们已经对动物双歧杆菌亚种的两个同基因菌株的基因组进行了测序。产生EPS的表型不同的乳酸菌。原始菌株显示出非粘液样外观，并且其衍生的突变体已经获得了粘液型表型。他们的基因组的序列分析显示Balat_1410基因中的一个非同义突变，可能与EPS链的延长有关。通过比较已删除该基因的菌株与含有野生型和突变基因的菌株，我们能够证明每种菌株显示出不同的细胞表面特征。由在Balat_1410中携带突变的菌株合成的粘液EPS提供了对胃肠道条件的更高抵抗力，并增加了对人肠上皮细胞的粘附能力。另外，人外周血单核细胞和离体结肠组织的细胞因子谱表明，粘液样菌株可能具有更高的抗炎活性。我们的发现提供了有关Balat_1410功能的相关数据，并揭示了粘液表型能够改变细胞中一些最相关的功能特性。

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