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首页> 外文期刊>Applied Microbiology >The Salmonella Transcriptome in Lettuce and Cilantro Soft Rot Reveals a Niche Overlap with the Animal Host Intestine
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The Salmonella Transcriptome in Lettuce and Cilantro Soft Rot Reveals a Niche Overlap with the Animal Host Intestine

机译：生菜和香菜软腐中的沙门氏菌转录组揭示了与动物宿主肠道的利基重叠

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Fresh vegetables have been recurrently associated with salmonellosis outbreaks, and Salmonella contamination of retail produce has been correlated positively with the presence of soft rot disease. We observed that population sizes of Salmonella enterica serovar Typhimurium SL1344 increased 56-fold when inoculated alone onto cilantro leaves, versus 2,884-fold when coinoculated with Dickeya dadantii , a prevalent pathogen that macerates plant tissue. A similar trend in S. enterica populations was observed for soft-rotted lettuce leaves. Transcriptome analysis of S. enterica cells that colonized D. dadantii -infected lettuce and cilantro leaves revealed a clear shift toward anaerobic metabolism and catabolism of substrates that are available due to the degradation of plant cells by the pectinolytic pathogen. Twenty-nine percent of the genes that were upregulated in cilantro macerates were also previously observed to have increased expression levels in the chicken intestine. Furthermore, multiple genes induced in soft rot lesions are also involved in the colonization of mouse, pig, and bovine models of host infection. Among those genes, the operons for ethanolamine and propanediol utilization as well as for the synthesis of cobalamin, a cofactor in these pathways, were the most highly upregulated genes in lettuce and cilantro lesions. In S . Typhimurium strain LT2, population sizes of mutants deficient in propanediol utilization or cobalamin synthesis were 10- and 3-fold lower, respectively, than those of the wild-type strain in macerated cilantro ( P < 0.0002); in strain SL1344, such mutants behaved similarly to the parental strain. Anaerobic conditions and the utilization of nutrients in macerated plant tissue that are also present in the animal intestine indicate a niche overlap that may explain the high level of adaptation of S. enterica to soft rot lesions, a common postharvest plant disease.
机译：新鲜蔬菜经常与沙门氏菌病爆发有关，零售产品的沙门氏菌污染与软腐病呈正相关。我们观察到，单独接种到香菜叶上时，肠炎沙门氏菌血清型鼠伤寒沙门氏菌SL1344的种群大小增加了56倍，而与Dickeya dadantii（一种浸渍植物组织的普遍病原体）同时接种时，种群大小增加了2884倍。对于软腐生菜叶，在小肠链球菌种群中也观察到类似趋势。定居达达蒂尼感染的生菜和香菜叶的肠炎沙门氏菌细胞的转录组分析表明，由于果胶分解病原体对植物细胞的降解，基质向厌氧代谢和分解代谢的转变明显。先前还观察到在香菜浸软物中上调的基因中有29％在鸡肠中表达水平增加。此外，在软腐病中诱导的多个基因也参与小鼠，猪和牛宿主感染模型的定殖。在这些基因中，用于乙醇胺和丙二醇的操纵子以及用于合成这些途径中的辅因子钴胺素的操纵子是莴苣和香菜病害中上调程度最高的基因。在S中。鼠伤寒沙门氏菌菌株LT2，缺乏丙二醇利用或钴胺素合成的突变体的种群大小分别比浸软叶中的野生型菌株低10倍和3倍（P <0.0002）;在菌株SL1344中，这种突变体的表现与亲本菌株相似。在动物肠中也存在的无氧条件和浸软植物组织中养分的利用表明利基重叠，这可以解释肠炎沙门氏菌对软腐烂病害（一种常见的收获后植物病）的高度适应。

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