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首页> 外文期刊>Applied Microbiology >Role of Extracellular Structures of Escherichia coli O157:H7 in Initial Attachment to Biotic and Abiotic Surfaces
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Role of Extracellular Structures of Escherichia coli O157:H7 in Initial Attachment to Biotic and Abiotic Surfaces

机译：大肠杆菌O157：H7的细胞外结构在最初附着于生物和非生物表面上的作用

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Infection by human pathogens through the consumption of fresh, minimally processed produce and solid plant-derived foods is a major concern of the U.S. and global food industries and of public health services. Enterohemorrhagic Escherichia coli O157:H7 is a frequent and potent foodborne pathogen that causes severe disease in humans. Biofilms formed by E. coli O157:H7 facilitate cross-contamination by sheltering pathogens and protecting them from cleaning and sanitation operations. The objective of this research was to determine the role that several surface structures of E. coli O157:H7 play in adherence to biotic and abiotic surfaces. A set of isogenic deletion mutants lacking major surface structures was generated. The mutant strains were inoculated onto fresh spinach and glass surfaces, and their capability to adhere was assessed by adherence assays and fluorescence microscopy methods. Our results showed that filament-deficient mutants bound to the spinach leaves and glass surfaces less strongly than the wild-type strain did. We mimicked the switch to the external environment—during which bacteria leave the host organism and adapt to lower ambient temperatures of cultivation or food processing—by decreasing the temperature from 37°C to 25°C and 4°C. We concluded that flagella and some other cell surface proteins are important factors in the process of initial attachment and in the establishment of biofilms. A better understanding of the specific roles of these structures in early stages of biofilm formation can help to prevent cross-contaminations and foodborne disease outbreaks.
机译：通过食用新鲜的，最低限度加工的农产品和固体植物来源的食品，人类病原体的感染是美国和全球食品工业以及公共卫生服务的主要关注点。肠出血性大肠杆菌O157：H7是常见且有效的食源性病原体，可导致人类严重疾病。大肠杆菌O157：H7形成的生物膜可通过掩盖病原体并保护其免受清洁和卫生操作而促进交叉污染。这项研究的目的是确定大肠杆菌O157：H7的几种表面结构在粘附生物和非生物表面中的作用。产生了一组缺乏主要表面结构的等基因缺失突变体。将突变菌株接种到新鲜的菠菜和玻璃表面上，并通过粘附测定和荧光显微镜法评估其粘附能力。我们的结果表明，与野生型菌株相比，缺乏丝状突变体的菠菜叶和玻璃表面结合力更弱。我们通过将温度从37°C降低到25°C和4°C来模拟向外部环境的转换（在此期间细菌离开宿主生物并适应较低的种植或食品加工环境温度）。我们得出的结论是，鞭毛和其他一些细胞表面蛋白是初始附着过程和生物膜建立过程中的重要因素。更好地了解这些结构在生物膜形成的早期阶段的具体作用可以帮助防止交叉污染和食源性疾病暴发。

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