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Persistence of Infectious Shiga Toxin-Encoding Bacteriophages after Disinfection Treatments

机译：消毒处理后感染志贺菌毒素的噬菌体的持久性

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In Shiga toxin-producing Escherichia coli (STEC), induction of Shiga toxin-encoding bacteriophages (Stx phages) causes the release of free phages that can later be found in the environment. The ability of Stx phages to survive different inactivation conditions determines their prevalence in the environment, the risk of stx transduction, and the generation of new STEC strains. We evaluated the infectivity and genomes of two Stx phages (Φ534 and Φ557) under different conditions. Infectious Stx phages were stable at 4, 22, and 37°C and at pH 7 and 9 after 1 month of storage but were completely inactivated at pH 3. Infective Stx phages decreased moderately when treated with UV (2.2-log_(10) reduction for an estimated UV dose of 178.2 mJ/cm~(2)) or after treatment at 60 and 68°C for 60 min (2.2- and 2.5-log_(10) reductions, respectively) and were highly inactivated (3 log_(10)) by 10 ppm of chlorine in 1 min. Assays in a mesocosm showed lower inactivation of all microorganisms in winter than in summer. The number of Stx phage genomes did not decrease significantly in most cases, and STEC inactivation was higher than phage inactivation under all conditions. Moreover, Stx phages retained the ability to lysogenize E. coli after some of the treatments.
机译：在产生志贺毒素的大肠杆菌（STEC）中，诱导志贺毒素的噬菌体（Stx噬菌体）的诱导导致释放游离噬菌体，这些噬菌体随后可在环境中发现。 Stx噬菌体在不同灭活条件下生存的能力决定了它们在环境中的流行程度，stx转导的风险以及新STEC菌株的产生。我们评估了两种Stx噬菌体（Φ534和Φ557）在不同条件下的感染力和基因组。感染性Stx噬菌体在储存1个月后在4、22和37°C以及pH 7和9处稳定，但在pH 3时完全灭活。当用UV处理时，感染性Stx噬菌体适度减少（减少2.2-log_（10））估计紫外线剂量为178.2 mJ / cm〜（2））或在60和68°C处理60分钟后（分别减少2.2和2.5 loglog（10））并高度失活（3 log_（10 ））在1分钟内加入10 ppm的氯。中膜的测定表明，冬季所有微生物的灭活率均低于夏季。在大多数情况下，Stx噬菌体基因组数目没有显着减少，并且在所有条件下，STEC灭活都高于噬菌体灭活。此外，在某些处理后，Stx噬菌体保留了裂解大肠杆菌的能力。

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