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首页> 外文期刊>Applied Microbiology >Development of a Novel Selective and Differential Medium for the Isolation of Listeria monocytogenes
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Development of a Novel Selective and Differential Medium for the Isolation of Listeria monocytogenes

机译：分离李斯特菌的新型选择性差异培养基的开发

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A new medium (lecithin and levofloxacin [LL] medium) is described for the isolation of Listeria monocytogenes from food samples. LL medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase C (PI-PLC) and phosphatidylcholine-specific phospholipase C (PC-PLC) produced by L. monocytogenes . Levofloxacin is incorporated to inhibit the growth of microorganisms other than L. monocytogenes , especially Bacillus cereus , shown to possess PI-PLC and PC-PLC activities. L. monocyogenes produced white colonies with a halo on LL medium, whereas Listeria innocua appeared as white colonies without a halo. Levofloxacin at 0.20 mg/liter completely inhibited the growth of B. cereus , while the growth of L. monocytogenes was unaffected. In the second phase of the study, the sensitivity and the specificity of LL medium were compared to those of modified Oxford agar (MOX) and two chromogenic media (Brilliance Listeria agar and CHROMagar Listeria ), using a total of 250 food samples. From 200 unspiked food samples, the specificity of LL medium (96.0%) was superior to that of MOX (72.0%) and similar to the specificities of Brilliance Listeria agar (96.5%) and CHROMagar Listeria (94.5%). From 50 spiked food samples, LL medium and CHROMagar Listeria represented the highest sensitivities (96.0%), followed by Brilliance Listeria agar (92.0%) and MOX (54.0%). Also, LL medium showed the highest confirmation rate (98.8%), followed by Brilliance Listeria agar (98.7%), CHROMagar Listeria (98.3%), and MOX (52.0%). On the basis of its good specificity and cost effectiveness, LL medium is useful for the isolation of L. monocytogenes from food samples.
机译：描述了一种新的培养基（卵磷脂和左氧氟沙星[LL]培养基），用于从食品样品中分离单核细胞增生李斯特菌。 LL培养基包括大豆中的卵磷脂，用于检测单核细胞增生李斯特氏菌产生的磷脂酰肌醇特异性磷脂酶C（PI-PLC）和磷脂酰胆碱特异性磷脂酶C（PC-PLC）。加入左氧氟沙星可抑制单核细胞增生李斯特氏菌以外的微生物（特别是蜡状芽孢杆菌）的生长，这些微生物具有PI-PLC和PC-PLC活性。单核细胞增生李斯特菌在LL培养基上产生带有光环的白色菌落，而无病李斯特菌则表现为没有光环的白色菌落。 0.20 mg / L的左氧氟沙星完全抑制蜡状芽胞杆菌的生长，而单核细胞增生李斯特菌的生长不受影响。在研究的第二阶段，将LL培养基与改良牛津琼脂（MOX）和两种生色培养基（Brilliance Listeria琼脂和CHROMagar Listeria）的敏感性和特异性进行了比较，共使用250个食物样品。在200个未加标食品样本中，LL培养基的特异性（96.0％）优于MOX的特异性（72.0％），与Brilliance Listeria琼脂（96.5％）和CHROMagar Listeria的特异性（94.5％）相似。在50个加标食品样本中，LL培养基和CHROMagar李斯特菌的敏感度最高（96.0％），其次是Brilliance Listeria琼脂（92.0％）和MOX（54.0％）。此外，LL培养基的确证率最高（98.8％），其次是华晨李斯特菌琼脂（98.7％），CHROMagar李斯特菌（98.3％）和MOX（52.0％）。基于其良好的特异性和成本效益，LL培养基可用于从食品样品中分离出单核细胞增生李斯特菌。

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