...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Development of a Recombinant Escherichia coli Strain for Overproduction of the Plant Pigment Anthocyanin
【24h】

Development of a Recombinant Escherichia coli Strain for Overproduction of the Plant Pigment Anthocyanin

机译：开发用于植物色素花青素过量生产的重组大肠杆菌菌株

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Anthocyanins are water-soluble colored pigments found in terrestrial plants and are responsible for the red, blue, and purple coloration of many flowers and fruits. In addition to the plethora of health benefits associated with anthocyanins (cardioprotective, anti-inflammatory, antioxidant, and antiaging properties), these compounds have attracted widespread attention due to their promising potential as natural food colorants. Previously, we reported the biotransformation of anthocyanin, specifically cyanidin 3- O -glucoside (C3G), from the substrate (+)-catechin in Escherichia coli . In the present work, we set out to systematically improve C3G titers by enhancing substrate and precursor availability, balancing gene expression level, and optimizing cultivation and induction parameters. We first identified E. coli transporter proteins that are responsible for the uptake of catechin and secretion of C3G. We then improved the expression of the heterologous pathway enzymes anthocyanidin synthase (ANS) and 3- O -glycosyltransferase (3GT) using a bicistronic expression cassette. Next, we augmented the intracellular availability of the critical precursor UDP-glucose, which has been known as the rate-limiting precursor to produce glucoside compounds. Further optimization of culture and induction conditions led to a final titer of 350 mg/liter of C3G. We also developed a convenient colorimetric assay for easy screening of C3G overproducers. The work reported here constitutes a promising foundation to develop a cost-effective process for large-scale production of plant-derived anthocyanin from recombinant microorganisms.
机译：花青素是在陆地植物中发现的水溶性有色颜料，可导致许多花朵和水果的红色，蓝色和紫色着色。除了与花色苷有关的多种健康益处（心脏保护，抗炎，抗氧化剂和抗衰老特性）外，这些化合物还因其作为天然食品着色剂的潜力而备受关注。以前，我们报道了从大肠杆菌中的底物（+）-儿茶素中花色苷，特别是花青素3-O-葡萄糖苷（C3G）的生物转化。在目前的工作中，我们着手通过增强底物和前体的可用性，平衡基因表达水平以及优化培养和诱导参数来系统地提高C3G滴度。我们首先确定了大肠杆菌转运蛋白，这些蛋白负责儿茶素的摄取和C3G的分泌。然后，我们使用双顺反子表达盒改善了异源途径酶花色素苷合酶（ANS）和3-O-糖基转移酶（3GT）的表达。接下来，我们增加了关键前体UDP-葡萄糖的细胞内可用性，后者被称为生产葡萄糖苷化合物的限速前体。培养和诱导条件的进一步优化导致最终滴定度为350 mg / L C3G。我们还开发了一种方便的比色测定法，可轻松筛查C3G过量生产者。此处报道的工作为开发从重组微生物大规模生产植物衍生的花色苷的经济高效方法奠定了有希望的基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号