• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Dynamics of Dual Infection with Campylobacter jejuni Strains in Chickens Reveals Distinct Strain-to-Strain Variation in Infection Ecology
【24h】

Dynamics of Dual Infection with Campylobacter jejuni Strains in Chickens Reveals Distinct Strain-to-Strain Variation in Infection Ecology

机译：空肠弯曲杆菌双重感染鸡的动力学揭示了感染生态学中不同菌株间的差异。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although multiple genotypes of Campylobacter jejuni may be isolated from the same commercial broiler flock, little is known about the infection dynamics of different genotypes within individuals or their colonization sites within the gut. Single experimental infections with C. jejuni M1 (sequence type 137, clonal complex 45) and C. jejuni 13126 (sequence type 21, clonal complex 21) revealed that 13126 colonized the ceca at significantly higher levels. The dissemination and colonization sites of the two C. jejuni strains then were examined in an experimental broiler flock. Two 33-day-old broiler chickens were infected with M1 and two with 13126, and 15 birds were left unchallenged. Cloacal swabs were taken postinfection to determine the colonization and shedding of each strain. By 2 days postinfection (dpi), 8/19 birds were shedding M1 whereas none were shedding 13126. At 8 dpi, all birds were shedding both strains. At 18 dpi, liver and cecal levels of each isolate were quantified, while in 10 birds they also were quantified at nine sites throughout the gastrointestinal (GI) tract. 13126 was found throughout the GI tract, while M1 was largely restricted to the ceca and colon. The livers of 7/19 birds were culture positive for 13126 only. These data show that 13126 has a distinctly different infection biology than strain M1. It showed slower colonization of the lower GI tract but was more invasive and able to colonize at a high level throughout the GI tract. The finding that C. jejuni strains have markedly different infection ecologies within the chicken has implications for control in the poultry industry and suggests that the contamination risk of edible tissues is dependent on the isolate involved.
机译：尽管空肠弯曲菌的多种基因型可以从同一个商品肉鸡群中分离出来，但人们对个体内不同基因型或其在肠道内定植部位的感染动态知之甚少。空肠弯曲杆菌M1（序列类型137，克隆复合体45）和空肠弯曲杆菌13126（序列类型21，克隆复合体21）的单次实验感染显示13126在盲肠中定居水平较高。然后在实验性肉鸡群中检查了两个空肠弯曲杆菌菌株的传播和定殖位点。两只33日龄的肉鸡被M1感染，两只被13126感染，未受攻击的有15只鸡。感染后取泄殖腔拭子以确定每个菌株的定植和脱落。感染后第2天（dpi），有8/19只禽舍掉了M1，没有一只舍弃了M13。在8 dpi时，所有禽舍都掉了两种毒株。在18 dpi时，对每种分离物的肝脏和盲肠水平进行了定量，而在10只鸡中，它们也在整个胃肠道（GI）的9个部位也进行了定量。在整个胃肠道中发现了13126，而M1主要限于盲肠和结肠。 7/19只鸡的肝脏仅对13126只培养呈阳性。这些数据表明13126与菌株M1有明显不同的感染生物学。它显示出较低的胃肠道定植较慢，但更具侵入性，能够在整个胃肠道中高水平定植。空肠弯曲杆菌菌株在鸡内的感染生态学有明显不同，这一发现对家禽业的控制具有重要意义，并表明可食组织的污染风险取决于所涉及的分离物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号