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首页> 外文期刊>Applied Microbiology >MS2 Virus Inactivation by Atmospheric-Pressure Cold Plasma Using Different Gas Carriers and Power Levels
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MS2 Virus Inactivation by Atmospheric-Pressure Cold Plasma Using Different Gas Carriers and Power Levels

机译：使用不同气体载体和功率水平的大气压冷等离子体灭活MS2病毒

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In this study, airborne MS2 bacteriophages were exposed for subsecond time intervals to atmospheric-pressure cold plasma (APCP) produced using different power levels (20, 24, and 28 W) and gas carriers (ambient air, Ar-O_(2) [2%, vol/vol], and He-O_(2) [2%, vol/vol]). In addition, waterborne MS2 viruses were directly subjected to the APCP treatment for up to 3 min. MS2 viruses with and without the APCP exposure were examined by scanning electron microscopy (SEM), reverse transcription-PCR (RT-PCR), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Viral inactivation was shown to exhibit linear relationships with the APCP generation power and exposure time ( R ~(2) > 0.95 for all energy levels tested) up to 95% inactivation (1.3-log reduction) after a subsecond airborne exposure at 28 W; about the same inactivation level was achieved for waterborne viruses with an exposure time of less than 1 min. A larger amount of reactive oxygen species (ROS), such as atomic oxygen, in APCP was detected for a higher generation power with Ar-O_(2) and He-O_(2) gas carriers. SEM images, SDS-PAGE, and agarose gel analysis of exposed waterborne viruses showed various levels of damage to both surface proteins and their related RNA genes after the APCP exposure, thus leading to the loss of their viability and infectivity.
机译：在这项研究中，空气传播的MS2噬菌体在亚秒的时间间隔内暴露于使用不同功率水平（20、24和28 W）和气体载体（环境空气，Ar-O_（2）[ 2％，体积/体积]和He-O_（2）[2％，体积/体积]）。此外，水性MS2病毒直接经过APCP处理长达3分钟。通过扫描电子显微镜（SEM），逆转录PCR（RT-PCR）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检查了暴露于APCP和未暴露于APCP的MS2病毒。病毒灭活显示出与APCP产生功率和暴露时间呈线性关系（对于所有测试的能量水平，R〜（2）> 0.95）在28 W的空气传播亚秒后高达95％的灭活（降低1.3 log）。对于水性病毒，暴露时间少于1分钟的灭活水平大致相同。使用Ar-O_（2）和He-O_（2）气体载体可以检测到APCP中大量的活性氧物质（ROS），例如原子氧，以获得更高的发电功率。暴露的水性病毒的SEM图像，SDS-PAGE和琼脂糖凝胶分析表明，暴露于APCP后，表面蛋白及其相关RNA基因均受到不同程度的破坏，从而导致其活力和感染力丧失。

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