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首页> 外文期刊>Infection and immunity >Interaction of hepatitis B surface antigen (Australia antigen) with membrane vesicles of Pseudomonas aeruginosa.
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Interaction of hepatitis B surface antigen (Australia antigen) with membrane vesicles of Pseudomonas aeruginosa.

机译：乙型肝炎表面抗原（澳大利亚抗原）与铜绿假单胞菌的膜囊泡相互作用。

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A lysogenic strain of Pseudomonas aeruginosa was cultured from the dialysis fluid of a patient on chronic hemodialysis treatment whose blood contained hepatitis B surface antigen (HB8Ag). When this bacterium was incubated for 4 to 7 days with serum containing HB8Ag or with purified HB8Ag, a loss of the HB8Ag-specific immunological reactivity was observed. Bacteriophages can be induced from the isolated P. aeruginosa with mitomycin C; the phages, after purification on CsCl gradients, also lyse P. aeruginosa strain 25102 (ATCC). Subsequent to gradient centrifugation of the lysate, a fraction was found with a density around 1.40 g/ml that inactivated HB8Ag after a 4-h incubation at 37 C as determined by counterelectrophoresis and hemagglutination inhibition. The activity was not found in appreciable amounts in other gradient fractions. The electron microscope shows that the active fraction contains envelope vesicles of 45 to 60 nm in diameter. In spite of their loss in HB8Ag activity, the HB8Ag particles (22nm) appeared morphologically intact. These findings suggest that an enzyme(s) is present in the vesicle fraction which inactivates antigenic determinants on HB8Ag particles. Thus, the presence of these bacteria in environments such as feces, dialysis tanks, and contaminated drinking water may prevent the detection of HB8Ag.
机译：从接受慢性血液透析治疗的患者的透析液中培养铜绿假单胞菌的溶原菌株，该患者的血液中含有乙型肝炎表面抗原（HB8Ag）。当该细菌与含有HB8Ag的血清或纯化的HB8Ag孵育4至7天时，观察到HB8Ag特异性免疫反应性降低。可以用丝裂霉素C从分离的铜绿假单胞菌中诱导噬菌体;在CsCl梯度上纯化后，噬菌体也裂解铜绿假单胞菌菌株25102（ATCC）。在将裂解物进行梯度离心后，发现了一个密度约为1.40 g / ml的馏分，通过反电泳和血凝抑制作用测定，该馏分在37°C孵育4小时后使HB8Ag失活。在其他梯度馏分中未发现明显的活性。电子显微镜显示，活性部分包含直径为45至60 nm的包膜囊泡。尽管它们失去了HB8Ag活性，但HB8Ag颗粒（22nm）在形态上似乎是完整的。这些发现表明，囊泡部分中存在一种酶，可以使HB8Ag颗粒上的抗原决定簇失活。因此，这些细菌在粪便，透析池和受污染的饮用水等环境中的存在可能会阻止检测HB8Ag。

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