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首页> 外文期刊>Infection and immunity >Structure and Immunological Specificity of the Streptococcus mutans Group b Cell Wall Antigen
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Structure and Immunological Specificity of the Streptococcus mutans Group b Cell Wall Antigen

机译：变形链球菌b族细胞壁抗原的结构和免疫学特异性

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The Streptococcus mutans group b antigen of strain FA1 has been defined as to chemical composition and immunological specificity. The antigen in cold trichloroacetic acid extracts was fractionated on diethylaminoethyl-Sephadex A-25 at pH 8.5. Two forms were isolated: a polysaccharide and a mucoprotein. The two polymers reacted as a single substance in agar gel diffusion against specific adsorbed FA1 rabbit antisera but were separated by gel immunoelectrophoresis. No reaction with any other S. mutans or streptococcal group sera occurred. Galactose composed about one-third and galactosamine about 3% of the total weight of each polymer. Rhamnose was a major component of the polysaccharide (47%) but was present only in traces in the mucoprotein. The protein content of the latter was about 40%. No significant quantities of glycerol, phosphorus, or muramic acid were present in either case. Pepsin and trypsin had no effect on the serological specificity of the mucoprotein. d-Galactose and d-galactosamine were strong inhibitors (70%) of the precipitin reaction, whereas d-glucose, d-glucosamine, and N-acetyl-d-glucosamine inhibited between 25 and 35%. The results indicate that the antigen is a major antigenic component of the cell wall and that the specificity of the antigen resides in binding sites which contain both d-galactose and d-galactosamine. Agglutination of whole cells by specific group b antiserum indicates the antibody receptor sites of the polysaccharide antigen are at the surface of the streptococcal cell. The mucoprotein, but not the polysaccharide, was released from the cell by lysozyme. Lysis did not occur. The immunological specificity and other characteristics of the antigen establishes it as the identifying antigen of S. mutans group b.
机译：已经确定了FA1菌株的变形链球菌组b抗原的化学组成和免疫学特异性。将冷的三氯乙酸提取物中的抗原在pH 8.5的二乙氨基乙基-Sephadex A-25上分级分离。分离出两种形式：多糖和粘蛋白。两种聚合物在琼脂凝胶扩散中作为单一物质与特异性吸附的FA1兔抗血清反应，但通过凝胶免疫电泳分离。与任何其他 S没有反应。发生了变形或链球菌血清。半乳糖占每种聚合物总重量的约三分之一，半乳糖胺约占每种聚合物总重量的3％。鼠李糖是多糖的主要成分（47％），但仅存在于粘蛋白中。后者的蛋白质含量为约40％。在这两种情况下，均不存在大量的甘油，磷酸或山梨酸。胃蛋白酶和胰蛋白酶对粘蛋白的血清学特异性没有影响。 d-半乳糖和d-半乳糖胺是沉淀素反应的强抑制剂（70％），而d-葡萄糖，d-葡萄糖胺和 N -乙酰基-d-葡萄糖胺抑制率在25％至35％之间。结果表明该抗原是细胞壁的主要抗原成分，并且该抗原的特异性在于包含d-半乳糖和d-半乳糖胺的结合位点。特定的b组抗血清对全细胞的凝集表明多糖抗原的抗体受体位点在链球菌细胞的表面。溶菌酶从细胞中释放出黏蛋白而不是多糖。裂解没有发生。抗原的免疫学特异性和其他特征将其确立为 S的鉴定抗原。 mutans 组b。

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