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首页> 外文期刊>Infection and immunity >Comparison of Immunofluorescence and Isolation Techniques in the Diagnosis of Respiratory Viral Infections of Children
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Comparison of Immunofluorescence and Isolation Techniques in the Diagnosis of Respiratory Viral Infections of Children

机译：免疫荧光和隔离技术在儿童呼吸道病毒感染诊断中的比较

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The immunoflourescent antibody technique (IFAT) and cell culture isolation procedures were compared for their efficiency in the etiological diagnosis of viral respiratory illness in children. Before the IFAT was incorporated as a routine procedure, antisera used in the test were carefully calibrated to insure specificity. A study was then conducted in which 375 nasopharyngeal suctions were investigated by both IFAT and isolation for the presence of parainfluenza virus types 1, 2, and 3, respiratory syncytial, influenza A, and influenza B viruses. Methods already established in our hospital for patient management and specimen collection were not altered for the purposes of the study. The IFAT, as conventionally practiced in the detection of respiratory virus antigens, requires adequate numbers of ciliated epithelial cells. There were 68.5% specimens which contained cells suitable for IFAT, whereas 31.5% had either an insufficient number or inappropriate types of cells and could be used only for virus isolation. Cell-associated immunoglobulins were detected in 16% of those specimens with adequate cells. When all specimens were considered regardless of their cell population, IFAT was inferior to isolation in diagnostic efficiency. However, isolation complemented by IFAT resulted in a statistically significant increase in number of positive virus identifications. Under routine working conditions in a large pediatric hospital, it was found that IFAT could not replace isolation techniques but could, if used in conjunction with isolation, provide a significant overall increase in number of positive diagnoses. The time that the specimen was taken in relation to first symptoms was found to be an important variable with respect to the method most likely to succeed in virus identification.
机译：比较了免疫荧光抗体技术（IFAT）和细胞培养分离程序在儿童病毒性呼吸道疾病的病因学诊断中的效率。在将IFAT纳入常规程序之前，应仔细校准测试中使用的抗血清以确保特异性。然后进行了一项研究，其中通过IFAT和隔离检查了375次鼻咽吸出，以检查是否存在1型，2型和3型副流感病毒，呼吸道合胞病毒，甲型流感病毒和乙型流感病毒。在本研究中，我们医院中已经建立的用于患者管理和标本采集的方法没有改变。如常规在检测呼吸道病毒抗原中所实践的那样，IFAT需要足够数量的纤毛上皮细胞。有68.5％的标本包含适合IFAT的细胞，而31.5％的标本细胞数量不足或类型不合适，只能用于病毒分离。在那些有足够细胞的标本中，有16％检测到了细胞相关的免疫球蛋白。当考虑所有标本而不考虑其细胞数量时，IFAT在诊断效率上不如孤立。但是，由IFAT补充的隔离导致阳性病毒鉴定数量的统计上显着增加。在一家大型儿科医院的常规工作条件下，人们发现IFAT不能代替隔离技术，但如果与隔离结合使用，可以显着增加阳性诊断的总体数量。相对于最有可能成功鉴定病毒的方法，发现与最初症状有关的标本采集时间是一个重要的变量。

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