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首页> 外文期刊>Infection and immunity >Studies on gonococcus infection. VIII. 125Iodine labeling of gonococci and studies on their in vitro interactions with eukaryotic cells.
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Studies on gonococcus infection. VIII. 125Iodine labeling of gonococci and studies on their in vitro interactions with eukaryotic cells.

机译：淋球菌感染的研究。八。 125淋球菌的碘标记及其在体外与真核细胞相互作用的研究。

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Intact gonococci (GC) have been labeled with 125iodine by the lactoperoxidase plus hydrogen peroxide procedure. The specific activities of types 2, 4, and 4 GC have been determined and are found to show small differences as follows: T4 greater than T2 greater than T4. 125I-labeled GC have been studied for their associations with both leukocytes and tissue culture cells. 125I-labeled GCshow the following relative order of association with the leukocytes: T2 equals T4* greater than T4. This contrasts with the relative degree of interaction between the GC and tissue culture cells, which follows the relative order: T2 greater than T4 equals T4*. Trypsin pretreatment of GC markedly reduces the association of all three types (T2, T4 AND T4*) with leukocytes but does not alter the level of attachment of any of the gonococcal types with tissue culture cells.
机译：完整的淋球菌（GC）已通过乳过氧化物酶加过氧化氢的程序标记为125碘。已经确定了类型2、4和4 GC的比活度，发现它们的细微差异如下：T4大于T2大于T4。已经研究了125 I标记的GC与白细胞和组织培养细胞的关联。 125 I标记的GC显示与白细胞关联的以下相对顺序：T2等于T4 *大于T4。这与GC和组织培养细胞之间的相对相互作用程度相反，后者遵循相对顺序：T2大于T4等于T4 *。胰蛋白酶对GC的预处理显着降低了所有三种类型（T2，T4和T4 *）与白细胞的关联，但没有改变任何淋球菌类型与组织培养细胞的附着水平。

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