• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Studies on Gonococcus Infection V. Observations on In Vitro Interactions of Gonococci and Human Neutrophils
【24h】

Studies on Gonococcus Infection V. Observations on In Vitro Interactions of Gonococci and Human Neutrophils

机译：淋球菌感染的研究V.淋球菌与人类嗜中性粒细胞体外相互作用的观察

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The association of in vitro human leukocytes with pilated, type 2 Neisseria gonorrhoeae exceeds that for nonpilated, type 4 organisms but is less than that for nonpilated, type 4* gonococci. The two nonpilated forms of gonococci (types 4 and 4*) attach to tissue culture cells to a much lesser extent than do pilated, type 2 organisms of the same strain. Trypsin treatment of either pilated (type 2) or nonpilated (type 4*) gonococci markedly reduces the attachment-ingestion of these organisms with leukocytes, but the same trypsin treatment does not depilate the type 2 organisms nor visibly alter the morphology of their pili. Similar reductions in association with leukocytes are found if the gonococci are pretreated with chymotrypsin, heat, or glutaraldehyde. High levels of association between gonococci and leukocytes are reestablished if the trypsin or chymotrypsin-treated organisms are reincubated in protease-free medium. These data suggest that interactions between gonococci and human neutrophils are mediated through surface characteristics of the bacteria, different from those which influence attachment of the organisms to tissue culture cells. In the latter instance, pili appear to positively influence gonococcal attachment, whereas in the former a nonpilus bacterial cell wall nonpilus protein is probably the major determiner in the interaction between leukocytes and gonococci.
机译：体外人类白细胞与2型非淋病奈瑟菌淋病奈瑟菌的结合超过了非4型非菌生菌，但小于4型非苏打性淋球菌。两种非去球形式的淋球菌（4型和4 * 型）附着在组织培养细胞上的程度远小于同一菌株的2型起球菌。用胰蛋白酶处理过毛的（2型）或未去绒毛的（4 * ）淋球菌明显减少了这些生物与白细胞的附着-摄入，但是相同的胰蛋白酶处理并不能使2型生物脱毛，也没有明显的作用。改变菌毛的形态。如果用糜蛋白酶，热或戊二醛预处理淋球菌，则会发现与白细胞相关的类似减少。如果将胰蛋白酶或经胰凝乳蛋白酶处理的生物体在无蛋白酶的培养基中重新孵育，则可重新建立淋球菌和白细胞之间的高水平缔合。这些数据表明，淋球菌和人类嗜中性粒细胞之间的相互作用是通过细菌的表面特征介导的，这不同于那些影响生物体附着于组织培养细胞的相互作用。在后一种情况下，菌毛似乎对淋球菌的附着有积极影响，而在前一种情况下，非菌毛细菌细胞壁的非菌毛蛋白可能是白细胞与淋球菌相互作用的主要决定因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号