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Mechanism of Action of Isoniazid on Mycobacterium bovis Strain BCG

机译：异烟肼对牛分枝杆菌卡介苗的作用机理

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The mechanism of action of isoniazid (INH) on Mycobacterium bovis strain BCG was studied. The rates of synthesis of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein after the addition of INH to growing cultures were followed by measuring the incorporation of 3H-thymidine, 3H-uridine, and 14C-l-valine, respectively. After the addition of INH, the rate of DNA synthesis began to decrease and was abolished within 4 hr. RNA synthesis ceased after 6 hr, and protein synthesis was inhibited after 7 hr. Thus, it appears that inhibition of the synthesis of DNA is one of the earliest events after INH addition. The inhibition of the synthesis of DNA was further found to correspond to losses in viability of treated cultures. Degradation of preexisting DNA in INH-treated strain BCG was not detected.
机译：研究了异烟肼（INH）对牛分枝杆菌BCG菌株的作用机理。在生长的培养物中加入INH后，测定脱氧核糖核酸（DNA），核糖核酸（RNA）和蛋白质的合成速率，然后测量 3 H-胸苷， 3的掺入率 H-尿苷和 14 Cl-缬氨酸。加入INH后，DNA合成速率开始降低，并在4小时内取消。 6小时后RNA合成停止，7小时后蛋白合成被抑制。因此，似乎抑制DNA的合成是加入INH后最早的事件之一。进一步发现抑制DNA合成对应于处理过的培养物的活力丧失。在INH处理的BCG菌株中未检测到预先存在的DNA降解。

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