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首页> 外文期刊>Infection and immunity >Factors affecting filamentation in Candida albicans: relationship of the uptake and distribution of proline to morphogenesis.
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Factors affecting filamentation in Candida albicans: relationship of the uptake and distribution of proline to morphogenesis.

机译：影响白色念珠菌丝化的因素：脯氨酸的摄取和分布与形态发生的关系。

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摘要

When glucose was present in high concentration, Candida albicans formed filaments in a phosphate-buffered medium, regardless of the nitrogen source. In lower concentrations of glucose, filamentation occurred only when various members of the glutamate, succinyl, or acetoacetyl-coenzyme A families of amino acids were used as sole nitrogen sources. Yeast morphology could be maintained either by replacing the amino acids in the medium with ammonium chloride or by making the medium high in phosphate or biotin. Studies using [U-14C]proline indicated that proline was catabolized in a manner consistent with the generation of increased cellular reducing potential and that the proline label entered into the Kreb's cycle. A reduction in Kreb's cycle activity was evidenced by an initial increase and then a rapid drop of the total organic acid content of the cells as well as in specific Kreb's cycle intermediates. Filamentation under conditions of low phosphate, high glucose, and increased cellular reduction potential, accompanied by a decrease in Kreb's cycle activity, suggests that morphogenesis in C. albicans is correlated with a Crabtree-like effect, i.e., repression of mitochondrial activity.
机译：当葡萄糖以高浓度存在时，白色念珠菌在磷酸盐缓冲液中形成细丝，而与氮源无关。在较低的葡萄糖浓度下，仅当将谷氨酸，琥珀酰或乙酰乙酰辅酶A的各种氨基酸作为唯一氮源时，才会发生丝状化。可以通过用氯化铵替代培养基中的氨基酸或通过使培养基中的磷酸盐或生物素含量高来维持酵母的形态。使用[U-14C]脯氨酸的研究表明脯氨酸以与增加的细胞还原潜能一致的方式分解代谢，并且脯氨酸标记进入克雷布氏循环。 Kreb循环活性的降低由细胞的总有机酸含量先增加然后迅速降低以及特定Kreb循环中间体证明。在低磷酸盐，高葡萄糖和增加的细胞还原潜能的条件下进行长丝化，同时伴随Kreb循环活性的降低，表明白色念珠菌的形态发生与Crabtree样效应相关，即线粒体活性的抑制。

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