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首页> 外文期刊>Infection and immunity >Suppressor T-cells in BCG-infected mice.
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Suppressor T-cells in BCG-infected mice.

机译：BCG感染小鼠中的抑制性T细胞。

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Specific pathogen-free B6D2 hybrid mice were infected with high (10(8) cells, intravenous), moderate (10(6) cells, intravenous), and low 10(3) cells, aerogenic) doses of viable BCG Pasteur. The growth of the BCG in the lungs and spleens of the three groups was followed over a 90-day period and correlated with the level of tuberculin hypersensitivity. Spleen cells were harvested from the three groups of mice at increasing time intervals and filtered through nylon wool to remove adherent cells, and the level of blast transformation after exposure to phytohemagglutinin and purified protein derivative was determined. Early in the BCG infection both the high- and the intermediate-dose groups showed enhanced thymidine incorporation by the spleen cell cultures, followed by a profound depression late in the infection. At this time, both groups of mice were anergic to purified protein derivative injected into footpads. Cell mixing studies demonstrated the presence of a population of suppressor cells in the spleens of the anergic animals. The suppressive abilities of these cells would be ablated by treatment with anti-Thy-1 antiserum and complement. The aerogenically infected mice were unresponsive to purified protein derivative but showed no evidence of suppressor T-cells. The lack of tuberculin sensitivity in these mice seemed to be due to a lack of sensitized T-cells in the spleen rather than to active immunosuppression.
机译：特定的无病原体B6D2杂种小鼠感染了高剂量（10（8）细胞，静脉内），中度（10（6）细胞，静脉内）和低10（3）细胞，有氧）剂量的可行BCG Pasteur。三天后，三组肺和脾中BCG的生长情况持续了90天，并与结核菌素超敏反应水平相关。以增加的时间间隔从三组小鼠中收获脾细胞，并通过尼龙毛过滤以除去粘附细胞，并测定暴露于植物血凝素和纯化的蛋白衍生物后胚细胞转化的水平。在BCG感染的早期，高剂量组和中等剂量组均显示脾细胞培养物中胸腺嘧啶核苷的掺入增加，随后在感染后期出现严重的抑郁症。这时，两组小鼠对注射到脚垫中的纯化蛋白衍生物均无反应。细胞混合研究表明，在厌氧动物的脾脏中存在抑制细胞群。这些细胞的抑制能力将通过抗Thy-1抗血清和补体的治疗而消除。受到气源感染的小鼠对纯化的蛋白衍生物无反应，但未显示抑制性T细胞的迹象。这些小鼠中结核菌素敏感性的缺乏似乎是由于脾脏中缺乏致敏的T细胞，而不是由于主动的免疫抑制。

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