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首页> 外文期刊>Infection and immunity >Classification of Neisseria meningitidis Group B into Distinct Serotypes II. Extraction of Type-Specific Antigens for Serotyping by Precipitin Techniques
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Classification of Neisseria meningitidis Group B into Distinct Serotypes II. Extraction of Type-Specific Antigens for Serotyping by Precipitin Techniques

机译：脑膜炎奈瑟氏球菌B组分为不同的血清型II。沉淀技术提取用于血清分型的特定类型抗原

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摘要

Over 10 distinct serotypes of group B Neisseria meningitidis have been found to date by using a sensitive microbactericidal assay developed by the authors. The serotype antigens have now been extracted by hot acid or saline extraction procedures. It was found that these extracted serotype antigens may be used in a simple capillary precipitin method. This method uses unadsorbed, undiluted rabbit antisera. In the capillary precipitin method a 3+ to 4+ reaction was considered significant. The microbactericidal assay and capillary precipitin methods for serotyping group B meningococci show excellent agreement. Group B meningococci were also serotyped by an agar gel double diffusion technique. The latter technique conserves reagents and has the further advantage that it does not show the minor cross-reactions observed in the capillary precipitin method. Thus two simple, reproducible methods for serological typing of group B meningococci have been developed. These methods developed for the serological typing of group B meningococci will aid in epidemiological studies of meningococcal disease. They may also be of value for selecting suitable strains for vaccine production.
机译：迄今为止，通过使用作者开发的敏感的杀菌方法，已发现B组脑膜炎奈瑟氏球菌的10种不同血清型。现在已经通过热酸或盐水提取程序提取了血清型抗原。已发现这些提取的血清型抗原可用于简单的毛细管沉淀蛋白方法。该方法使用未吸附，未稀释的兔抗血清。在毛细管沉淀法中，认为3+至4+反应很重要。 B组脑膜炎球菌血清分型的微细菌测定和毛细管沉淀法显示出极好的一致性。还通过琼脂凝胶双扩散技术对B组脑膜炎球菌进行血清分型。后一种技术节省了试剂，并具有另一个优点，即它没有显示出在毛细管沉淀法中观察到的较小的交叉反应。因此，已经开发出两种简单的，可重复的B组脑膜炎球菌血清学分型方法。这些为B组脑膜炎球菌进行血清学分型而开发的方法将有助于脑膜炎球菌疾病的流行病学研究。它们对于选择合适的疫苗生产菌株也可能有价值。

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