Secretion of phospholipase C by Pseudomonas aeruginosa.

M W Stinson; C Hayden

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Secretion of phospholipase C by Pseudomonas aeruginosa.

机译：铜绿假单胞菌分泌磷脂酶C。

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The conditions necessary for the secretion of phospholipase C (phosphatidylcholine cholinephosphohydrolase) by Pseudomonas aeruginosa were studied. Enzyme secretion by washed cell suspensions required a carbon source and ammonium, potassium, and calcium ions. The calcium requirement could be substituted by magnesium and strontium but not by copper, manganese, cobalt, or zinc. During growth in liquid medium, cells secreted phospholipase C during late logarithmic and early stationary phases. Secretion was repressed by the addition of inorganic phosphate but not by organic phosphates, glucose, or sodium succinate. Studies with tetracycline indicated that de novo protein synthesis was necessary for the secretion of phospholipase C and that the exoenzyme was not released from a preformed periplasmic pool. Similarly, extraction of actively secreting cells with 0.2 M MgCl2 at pH 8.4 solubilized large quantities of the periplasmic enzyme alkaline phosphatase but insignificant amounts of phospholipase C. Bacteria continued to secrete enzyme for nearly 45 min after the addition of inorganic phosphate or rifampin.

机译：研究了铜绿假单胞菌分泌磷脂酶C（磷脂酰胆碱胆碱磷酸水解酶）的必要条件。洗涤过的细胞悬液分泌的酶需要碳源以及铵，钾和钙离子。钙需要量可以用镁和锶代替，但不能用铜，锰，钴或锌代替。在液体培养基中生长期间，细胞在对数后期和静止初期分泌磷脂酶C。通过添加无机磷酸盐抑制分泌，但不通过有机磷酸盐，葡萄糖或琥珀酸钠抑制分泌。用四环素进行的研究表明，从头合成蛋白质是分泌磷脂酶C所必需的，并且外切酶没有从预先形成的周质池中释放。同样，在pH 8.4下用0.2 M MgCl2提取活跃分泌的细胞可溶解大量的周质酶碱性磷酸酶，但微不足道的磷脂酶C。细菌在加入无机磷酸盐或利福平后持续分泌酶近45分钟。

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《Infection and immunity》 |1979年第2期|共7页
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M W Stinson; C Hayden;
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1. PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa. [J] . A Cota-Gomez, A I Vasil, J Kadurugamuwa, Infection and immunity . 1997,第7期

机译：PlcR1和PlcR2是分泌铜绿假单胞菌溶血磷脂酶C所需的钙结合蛋白。
2. Identification of a new phospholipase C activity by analysis of an insertional mutation in the hemolytic phospholipase C structural gene of Pseudomonas aeruginosa. [J] . R M Ostroff, M L Vasil Journal of bacteriology . 1987,第10期

机译：通过分析铜绿假单胞菌溶血磷脂酶C结构基因中的插入突变来鉴定新的磷脂酶C活性。
3. End-products diacylglycerol and ceramide modulate membrane fusion induced by a phospholipase C/sphingomyelinase from Pseudomonas aeruginosa. [J] . Ibarguren M, Bomans PH, Frederik P Biochimica et biophysica acta. Biomembranes . 2010,第1期

机译：终产物二酰基甘油和神经酰胺调节铜绿假单胞菌的磷脂酶C /鞘磷脂酶诱导的膜融合。
4. Synthesis and Characterization of Biogenic Selenium Nanoparticles with Antimicrobial Properties Made by Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Pseudomonas aeruginosa. [C] . David Medina, Thomas J. Webster Society for Biomaterials annual meeting and exposition . 2018

机译：金黄色葡萄球菌，耐甲氧西林金黄色葡萄球菌（MRSA），大肠杆菌和铜绿假单胞菌制成的具有抗菌特性的生物硒纳米粒子的合成与表征。
5. The biochemical characterization and cell biology of the hemolytic phospholipase C of Pseudomonas aeruginosa. [D] . Stonehouse, Martin Joseph. 2004

机译：铜绿假单胞菌溶血磷脂酶C的生化特性和细胞生物学。
6. PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa. [O] . A Cota-Gomez, A I Vasil, J Kadurugamuwa, 1997

机译：PlcR1和PlcR2是铜绿假单胞菌溶血磷脂酶C分泌所需的假定钙结合蛋白。
7. Identification of a new phospholipase C activity by analysis of an insertional mutation in the hemolytic phospholipase C structural gene of Pseudomonas aeruginosa. [O] . Ostroff, R M, Vasil, M L 1987

机译：通过分析铜绿假单胞菌溶血磷脂酶C结构基因中的插入突变来鉴定新的磷脂酶C活性。

Secretion of phospholipase C by Pseudomonas aeruginosa.

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