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首页> 外文期刊>Infection and immunity >In Vitro Stimulation of Hamster Lymphocytes with Concanavalin A
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In Vitro Stimulation of Hamster Lymphocytes with Concanavalin A

机译：伴刀豆球蛋白A体外刺激仓鼠淋巴细胞

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The in vitro response of hamster lymphocytes to concanavalin A (Con A) was investigated by using the 3H-thymidine incorporation assay, and the results obtained were compared to those with phytohemagglutinin (PHA-P). The optimum culture conditions for stimulation were obtained when one million lymph node cells were cultivated for 72 hr in 2 ml of RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum in the presence of either 2 μg of Con A or 0.05 ml of PHA-P. The average ratios of stimulation using lymph node cells with Con A and PHA-P were 227 and 14, respectively. Bone marrow and thymus cells responded very poorly or not at all. Methyl α-d-glucopyranoside, when added to the culture medium at the time of addition of Con A, completely inhibited stimulation. The lymphocyte stimulation could be reversed by addition of the sugar as late as 18 hr after the addition of Con A. The lymphocyte response from hamsters bearing tumors induced by simian virus 40 tumor cells and from hamsters immune to tumor transplants was comparable to the response of lymphocytes from healthy donors.
机译：采用 3 H-胸苷掺入法研究了仓鼠淋巴细胞对伴刀豆球蛋白A（Con A）的体外反应，并将所得结果与植物血凝素（PHA-P）进行了比较。当在2μgCon A或0.05 ml存在下，在2 ml补充有10％热灭活胎牛血清的RPMI-1640培养基中培养一百万个淋巴结细胞72小时，从而获得刺激的最佳培养条件。 PHA-P。使用带有Con A和PHA-P的淋巴结细胞的平均刺激率分别为227和14。骨髓和胸腺细胞反应非常差或根本没有反应。当在添加Con A时将甲基α-d-吡喃葡萄糖苷添加到培养基中时，完全抑制了刺激。加入Con A后18小时可以通过加入糖来逆转淋巴细胞的刺激作用。猿猴病毒40肿瘤细胞诱导的携带肿瘤的仓鼠的淋巴细胞反应和免疫肿瘤移植的仓鼠的淋巴细胞反应可以与HBV的反应媲美。来自健康捐献者的淋巴细胞。

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