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首页> 外文期刊>Infection and immunity >Isolation and immunochemical characterization of the group-specific antigen of Streptococcus mutants 6715.
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Isolation and immunochemical characterization of the group-specific antigen of Streptococcus mutants 6715.

机译：链球菌突变体6715的组特异性抗原的分离和免疫化学表征。

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The group d antigen of Streptococcus mutans 6515 was isolated from a buffer (pH 7.3)-boiled extract of whole cells and analyzed immunochemically. Rabbits immunized in three different fashions with whole S. mutans 6715 each responded to the same antigenic cell surface component. This presumptive major antigen was found in culture supernatant, sonically treated supernatant, acid and buffer extracts of whole cells, and trichloroacetic acid extract of cell membranes. A crude preparation of this antigen could completely inhibit antibody-mediated cell (S. mutans 6715) agglutination in a spectrophotometric analysis. The antigen was purified from buffer-boiled extracts by gel filtration on columns of Sepharose 4B. The antigen did not migrate to the anode on electrophoresis nor did it contain appreciable quantities of phosphorus, glycerol, or ribitol. This suggested that the d antigenicity did not reside in a teichoic acid. The d antigen contained galactose and glucose as the sole saccharides, in a ratio of 5.9:1.0. Protein (9.5%) appeared to be a portion of the antigen, although Pronase-digested antigen retained the same electrophoretic mobility and could precipitate virtually all (98.6%) purified antibody directed to the intact antigen. The data obtained from hapten innvolved. Glucose also contributed to the immunodominant region. Antibody directed to the d antigen may be of importance in the inhibition of adherence phenomena manifested by S. mutans organisms of the d group.
机译：从煮沸的全细胞缓冲液（pH 7.3）中提取变形链球菌6515的D组抗原，并进行免疫化学分析。以三种不同方式用完整变形链球菌6715免疫的兔子对相同的抗原细胞表面成分有反应。在培养上清液，超声处理的上清液，全细胞的酸和缓冲液提取物以及细胞膜的三氯乙酸提取物中发现了这种推测的主要抗原。在分光光度分析中，这种抗原的粗制制剂可以完全抑制抗体介导的细胞（变形链球菌6715）凝集。通过在琼脂糖凝胶4B柱上的凝胶过滤从沸腾的提取物中纯化抗原。抗原在电泳时不会迁移到阳极，也不会包含大量的磷，甘油或核糖醇。这表明d抗原性不存在于硫磷酸中。 d抗原含有半乳糖和葡萄糖作为唯一糖，比率为5.9：1.0。蛋白质（9.5％）似乎是抗原的一部分，尽管被Pronase消化的抗原保留了相同的电泳迁移率，并且实际上可以沉淀出所有（98.6％）纯化的针对完整抗原的抗体。从半抗原获得的数据参与其中。葡萄糖也有助于免疫优势区域。针对d抗原的抗体在抑制由d组变形链球菌生物体表现出的粘附现象中可能是重要的。

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