...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Haemophilus influenzae adheres to and enters cultured human epithelial cells.
【24h】

Haemophilus influenzae adheres to and enters cultured human epithelial cells.

机译：流感嗜血杆菌粘附并进入培养的人上皮细胞。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Haemophilus influenzae is a common commensal organism of the human respiratory tract that initiates infection by colonizing the nasopharyngeal epithelium. In some individuals, colonization is followed by localized respiratory tract or systemic disease. To gain insight into the mechanisms by which H. influenzae attaches to and persists within the nasopharynx, we examined the interactions between a nonpiliated clinical isolate of H. influenzae and human epithelial cells. We noted substantial adherence that occurred independently of pili and required viable bacteria capable of de novo protein synthesis. Comparison of profiles of outer membrane proteins synthesized during incubation with epithelial cells for adherent and nonadherent bacteria identified several candidate adhesin molecules. In addition, a small number of adherent bacteria were capable of entering epithelial cells in a process that was inhibited by cytochalasin D and colchicine. The suggestion from our studies is that one or more of several newly synthesized nonpilus bacterial proteins are required for maximal in vitro adherence and invasion. We speculate that H. influenzae entry into epithelial cells may provide a mechanism for evasion of host defenses, thereby allowing persistence in the nasopharynx.
机译：流感嗜血杆菌是人类呼吸道的常见共生生物，其通过在鼻咽上皮中定殖而开始感染。在某些个体中，定植后是局部呼吸道或全身性疾病。为了深入了解流感嗜血杆菌附着并持续存在于鼻咽中的机制，我们检查了流感嗜血杆菌的非软化临床分离株与人上皮细胞之间的相互作用。我们注意到发生了独立于菌毛的大量粘附，并且需要能够从头合成蛋白质的活细菌。比较上皮细胞与粘附和非粘附细菌孵育过程中合成的外膜蛋白的概况，发现了几种候选粘附素分子。另外，少数粘附细菌能够在被细胞松弛素D和秋水仙碱抑制的过程中进入上皮细胞。我们研究的建议是，最大程度的体外粘附和侵袭需要几种新合成的非菌毛细菌蛋白中的一种或多种。我们推测流感嗜血杆菌进入上皮细胞可能提供逃避宿主防御的机制，从而使鼻咽中存在持久性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号