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首页> 外文期刊>Auris, nasus, larynx >Nontypeable Haemophilus influenzae isolated from intractable acute otitis media internalized into cultured human epithelial cells.
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Nontypeable Haemophilus influenzae isolated from intractable acute otitis media internalized into cultured human epithelial cells.

机译：从难治性急性中耳炎中分离出的不可分型流感嗜血杆菌内化到培养的人上皮细胞中。

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OBJECTIVES: The aim of this study is to examine the internalization of nontypeable Haemophilus influenzae (NTHi) into human epithelial cells. METHODS: Bactericidal assay was applied to examine the effects of antibiotics against cell-adherent NTHi using HEp-2 cells. A trans-well chamber assay was applied to examine the internalization and penetration of NTHi using Detroit562 cells. RESULTS: The adherence of NTHi to HEp-2 cells was noted after 2h of incubation. Azithromycin had a strong bactericidal effect against both cell-associated and non-adherent NTHi, while ceftriaxone did not show bactericidal effects on NTHi adhered to the HEp-2 cells. Three (60.0%) out of five NTHi isolates from the nasopharynx of children with intractable acute otitis media (AOM) internalized into and subsequently penetrated through the epithelial cells at various degrees. Azithromycin had a strong bactericidal effect against the cell-internalized NTHi, while ceftriaxone was bactericidal only against extracellular NTHi. CONCLUSION: The potential of NTHi as the intracellular pathogen may contribute to the persistent existence of this pathogen that result in the prolonged and intractable clinical course of AOM. Azithromycin may be a therapeutically significant antibiotic for patients with prolonged respiratory tract infections due to NTHi.
机译：目的：本研究的目的是检查不可分型流感嗜血杆菌（NTHi）进入人上皮细胞的内在化。方法：采用杀菌法检测HEp-2细胞对细胞粘附NTHi的抗菌作用。使用底特律562细胞，采用跨孔室分析法检查NTHi的内在化和穿透性。结果：孵育2h后，NTHi粘附于HEp-2细胞。阿奇霉素对与细胞相关的NTHi和对非粘附的NTHi都具有强的杀菌作用，而头孢曲松对粘附于HEp-2细胞上的NTHi没有杀菌作用。来自顽固性急性中耳炎（AOM）患儿鼻咽的5种NTHi分离物中，有3种（60.0％）内化并随后以不同程度渗透到上皮细胞中。阿奇霉素对细胞内在的NTHi具有很强的杀菌作用，而头孢曲松仅对细胞外的NTHi具有杀菌作用。结论：NTHi作为细胞内病原体的潜力可能有助于该病原体的持续存在，从而导致AOM的临床过程延长且难以治疗。阿奇霉素可能是因NTHi导致呼吸道感染延长的患者的重要治疗抗生素。

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