• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Cloning and expression of a species-specific early immunogenic 36-kilodalton protein of Mycoplasma hyopneumoniae in Escherichia coli.
【24h】

Cloning and expression of a species-specific early immunogenic 36-kilodalton protein of Mycoplasma hyopneumoniae in Escherichia coli.

机译：大肠杆菌中猪肺炎支原体物种特异性早期免疫原性36千达尔顿蛋白的克隆和表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mycoplasma hyopneumoniae, the etiologic agent of porcine enzootic pneumonia, synthesizes a 36-kDa protein which is an early and strong immunogenic factor in experimentally and naturally infected swine. The gene encoding this protein was cloned by screening a gene library of M. hyopneumoniae DNA with rabbit hyperimmune serum made against whole M. hyopneumoniae cells and convalescent-phase swine serum. Analysis of the recombinant protein expressed in Escherichia coli by immunoblot techniques showed that the protein is expressed in E. coli in its full length and does not cross-react with proteins from M. flocculare or M. hyorhinis. Genetic analysis showed that the gene was expressed from the lac promoter of the vector and seems to be translationally initiated from its own ribosome binding site. Subcloning in a transcriptional fusion vector to optimize expression resulted in production of the 36-kDa protein in E. coli at levels up to 30% of total protein.
机译：猪肺炎支原体肺炎支原体肺炎支原体，合成了一种36 kDa的蛋白质，该蛋白质是实验性和自然感染猪中的早期强免疫原性因子。通过用针对猪肺炎支原体完整细胞和恢复期猪血清制备的兔超免疫血清筛选猪肺炎支原体DNA的基因文库，克隆出编码该蛋白的基因。通过免疫印迹技术分析在大肠杆菌中表达的重组蛋白表明，该蛋白在大肠杆菌中全长表达，并且不会与絮状支原体或猪支原体的蛋白发生交叉反应。遗传分析表明，该基因是从载体的lac启动子表达的，似乎是从其自身的核糖体结合位点翻译起始的。亚克隆到转录融合载体中以优化表达，从而在大肠杆菌中产生了36 kDa的蛋白质，其含量高达总蛋白质的30％。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号