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首页> 外文期刊>Infection and immunity >Effect of methylamine and monodansylcadaverine on the susceptibility of McCoy cells to Chlamydia trachomatis infection.
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Effect of methylamine and monodansylcadaverine on the susceptibility of McCoy cells to Chlamydia trachomatis infection.

机译：甲胺和单丹酰尸胺对麦考伊细胞对沙眼衣原体感染的敏感性的影响。

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We used inhibitors of receptor-mediated endocytosis to study the mechanisms of infectivity, especially the uptake mechanism, of Chlamydia trachomatis for cultured cells. The effect of methylamine and monodansylcadaverine on the different stages of the chlamydial growth cycle in McCoy cells was examined. There was a dose-related decrease in the number of chlamydial inclusions in the presence of these agents. Monodansylcadaverine also decreased the chlamydia-dependent uptake of radioactive amino acids. The agents did not affect the attachment of chlamydiae to the cells, but they increased the protease-removable fraction of cell-bound chlamydiae. The amines reduced the number of inclusions when added at different times during the first 24 h of infection. However, this effect was influenced by host cell density, so that the effect of the amines at the early infectious phase was nullified in confluent monolayers, whereas, during later phases, the effect was comparatively independent of host cell density. This indicates that the amines have different modes of action at different infectious stages. The effect of the amines was reversible, and they had no effect on the infectivity of pretreated chlamydial elementary bodies. These experiments suggest that methylamine and monodansylcadaverine inhibit both the internalization of chlamydiae into McCoy cells and their intracellular development. These results are consistent with the hypothesis that chlamydiae utilize a constitutive cellular process, such as receptor-mediated endocytosis, to enter cells.
机译：我们使用受体介导的内吞作用抑制剂来研究沙眼衣原体对培养细胞的感染性机制，尤其是摄取机制。研究了甲胺和单丹磺酰尸胺对McCoy细胞衣原体生长周期不同阶段的影响。在存在这些药剂的情况下，衣原体包涵体的数量与剂量有关。 Monodansylcadaverine还减少了衣原体依赖的放射性氨基酸摄取。药剂不影响衣原体与细胞的附着，但是它们增加了细胞结合的衣原体中可去除蛋白酶的部分。当在感染的前24小时内的不同时间添加时，胺会减少夹杂物的数量。但是，该作用受宿主细胞密度的影响，因此在汇合单层中，胺在早期感染阶段的作用无效，而在后期阶段，该作用相对独立于宿主细胞密度。这表明胺在不同的感染阶段具有不同的作用方式。胺的作用是可逆的，并且对预处理的衣原体基本体的感染性没有影响。这些实验表明甲胺和单丹酰尸胺抑制衣原体进入McCoy细胞的内在化及其细胞内发育。这些结果与假衣原体利用组成型细胞过程（例如受体介导的内吞作用）进入细胞的假说相符。

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