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Dissemination of Listeria monocytogenesby Infected Phagocytes

机译：感染的吞噬细胞传播单核细胞增生李斯特菌

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In vitro data suggest that blood-borne Listeria monocytogenes organisms enter the central nervous system (CNS) by direct invasion of endothelial cells or by cell-to-cell spread from infected phagocytes to endothelial cells. However, a role for infected phagocytes in neuroinvasion and dissemination of L. monocytogenes in vivo has not been confirmed experimentally. Experiments described here tested whether L. monocytogenes-infected peripheral blood leukocytes (PBL) circulated in bacteremic mice and could establish organ infection in vivo. A mean of 30.5% of bacteria cultured from whole blood were PBL associated, and microscopy showed that 22.2% of monocytes and 1.6% of neutrophils were infected. PBL-associated bacteria spread to endothelial cells in vitro, indicating their potential for virulence in vivo. To test this possibility, mice were injected intravenously with infected PBL and CFU of bacteria in liver, spleen, and brain were quantified and compared with values for mice injected with broth-grown bacteria and in vitro-infected macrophage cell lines. An inoculum of infected macrophage cell lines led to greater numbers of bacteria in the liver than the numbers produced by a similar inoculum of broth-grown bacteria. In contrast, brain infection was best established by infected PBL. Results of intraperitoneal injection of infected peritoneal cells compared with results of injection with infected J774A.1 cells suggested that unrestricted intracellular bacterial replication within J774A.1 cells contributed to excessive liver infection in those mice. These data show dissemination of intracellularL. monocytogenes and indicate that phagocyte-facilitated invasion has a role in CNS infection in vivo. Heterogeneity with regard to bactericidal activity as well as to other phagocyte characteristics is a critical feature of this mechanism.
机译：体外数据表明，血源性单核细胞增生李斯特菌生物是通过直接入侵内皮细胞或从感染的吞噬细胞向内皮细胞扩散而进入中枢神经系统。然而，感染的吞噬细胞在神经浸润和 L传播中具有一定作用。体内单核细胞增生症尚未得到实验证实。此处描述的实验测试了 L。感染小鼠的单核细胞增生病感染的外周血白细胞（PBL）可以在体内建立器官感染。从全血中培养出的细菌平均有30.5％与PBL相关，并且显微镜检查显示22.2％的单核细胞和1.6％的中性粒细胞被感染。 PBL相关细菌在体外扩散到内皮细胞，表明它们在体内具有毒性。为了测试这种可能性，对小鼠静脉注射感染的PBL，并对肝，脾和脑中细菌的CFU进行定量，并与注射有肉汤生长细菌和体外感染的巨噬细胞系的小鼠的值进行比较。感染的巨噬细胞细胞系接种物在肝脏中的细菌数量要多于类似的肉汤生长细菌接种物所产生的细菌数量。相反，脑部感染最好由感染的PBL建立。腹膜内注射感染的腹膜细胞的结果与注射感染的J774A.1细胞的结果相比，表明J774A.1细胞内不受限制的细胞内细菌复制导致了这些小鼠的过度肝感染。这些数据表明细胞内emL的传播。并表明吞噬细胞促进的侵袭在体内中枢神经系统感染中起作用。关于杀菌活性以及其他吞噬细胞特性的异质性是该机制的关键特征。

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