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首页> 外文期刊>Infection and immunity >Chlamydial Development Is Adversely Affected by Minor Changes in Amino Acid Supply, Blood Plasma Amino Acid Levels, and Glucose Deprivation
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Chlamydial Development Is Adversely Affected by Minor Changes in Amino Acid Supply, Blood Plasma Amino Acid Levels, and Glucose Deprivation

机译：衣原体的发展受到氨基酸供应，血浆氨基酸水平和葡萄糖剥夺的微小变化的不利影响

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This study has demonstrated the extreme sensitivity ofChlamydia trachomatis growing in McCoy cells to small changes in external amino acid supply. In the absence of cycloheximide, a decrease in the amino acid concentration of medium to 75% of control values was sufficient to induce the growth of enlarged chlamydial forms of reduced infectivity. Morphology became more distorted and the yield of infectious particles from inclusions declined as medium amino acid levels were further reduced. These events correlated with a general decline in intracellular amino acids, as measured by high-performance liquid chromatography, suggesting that chlamydiae require a minimum concentration of each amino acid for normal development. Cycloheximide enhanced the production of normal organisms and increased infectivity yield in media, suggesting that the drug increased the available pool of amino acids. This was supported by intracellular amino acid analyses. Aberrant forms with reduced infectivity were also induced during supply of infected cell cultures with medium containing blood plasma amino acid concentrations, supporting the proposal that nutrient levels in vivo could promote abnormal chlamydial development. Markedly abnormal forms were also observed during glucose deprivation, providing further evidence that aberrant development is a general stress-related response.
机译：这项研究表明，在McCoy细胞中生长的沙眼衣原体对外部氨基酸供应量的微小变化具有极高的敏感性。在不存在环己酰亚胺的情况下，培养基的氨基酸浓度降低至对照值的75％足以诱导感染力降低的扩大的衣原体形式的生长。随着中等氨基酸水平的进一步降低，形态变得更加扭曲，夹杂物的传染性颗粒产率下降。这些事件与通过高效液相色谱法测定的细胞内氨基酸普遍下降有关，这表明衣原体需要最低浓度的每种氨基酸才能正常发育。环己二酰亚胺增强了正常生物的产生，并增加了培养基中的感染率，这表明该药物增加了氨基酸的可用量。细胞内氨基酸分析支持了这一点。在向感染的细胞培养物提供含有血浆氨基酸浓度的培养基的过程中，还诱导了感染性降低的异常形式，这支持了体内营养水平可能促进衣原体发育异常的提议。在葡萄糖剥夺期间还观察到明显异常形式，提供了进一步证据表明异常发育是与压力有关的一般反应。

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