Leukotriene B4 generation and DNA fragmentation induced by leukocidin from Staphylococcus aureus: protective role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF for human neutrophils.

T Hensler; B K?nig; G Prévost; Y Piémont; M K?ller; W K?nig

首页> 外文期刊>Infection and immunity >Leukotriene B4 generation and DNA fragmentation induced by leukocidin from Staphylococcus aureus: protective role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF for human neutrophils.

【24h】

Leukotriene B4 generation and DNA fragmentation induced by leukocidin from Staphylococcus aureus: protective role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF for human neutrophils.

机译：金黄色葡萄球菌中白三烯生成的白三烯B4生成和DNA片段化：粒细胞巨噬细胞集落刺激因子（GM-CSF）和G-CSF对人中性粒细胞的保护作用。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We studied the effect of leukocidin from Staphylococcus aureus V8 strains (Luk-PV) on the generation of Leukotriene B4 (LTB4) and its metabolites from human polymorphonuclear neutrophils (PMNs). Significant amounts of LTB4 were generated by PMNs after leukocidin exposure in a time- and dose-dependent manner, as shown by reversed-phase high-performance liquid chromatography analysis. In this regard, the S and F components of leukocidin acted synergistically. The calcium ionophore A23187 induced LTB4 generation, and the metabolism of exogenously added LTB4 into biologically less active omega-oxidated compounds was significantly decreased after leukocidin exposure. Priming of PMNs with granulocyte-macrophage colony-stimulating factor (GM-CSF) or G-CSF prior to leukocidin exposure substantially increased toxin- and calcium ionophore A23187-induced LTB4 formation. The inhibitory effects of leukocidin on mediator release were accompanied by membrane damage and DNA fragmentation, which were both restored after pretreatment with GM-CSF. The data suggest that the presence of costimulatory priming factors such as GM-CSF or G-CSF in the microenvironment of an inflammatory focus determines the pathophysiological effects induced by S. aureus leukocidin.

机译：我们研究了金黄色葡萄球菌V8株（Luk-PV）中白细胞介素对白三烯B4（LTB4）及其人类多形核中性粒细胞（PMNs）代谢产物的影响。如反相高效液相色谱分析所示，白细胞介素暴露后，PMN会以时间和剂量依赖性方式产生大量LTB4。在这方面，白血球菌素的S和F成分具有协同作用。钙离子载体A23187诱导了LTB4的生成，并且在暴露白介素后，外源添加的LTB4进入生物活性较低的ω-氧化化合物的代谢显着降低。在暴露白细胞抑制素之前，用粒细胞巨噬细胞集落刺激因子（GM-CSF）或G-CSF引发PMN会大大增加毒素和钙离子载体A23187诱导的LTB4的形成。白细胞抑制素对介质释放的抑制作用伴随着膜损伤和DNA断裂，在用GM-CSF预处理后均得以恢复。数据表明在炎症病灶的微环境中共刺激性启动因子如GM-CSF或G-CSF的存在决定了金黄色葡萄球菌白介素诱导的病理生理作用。

著录项

来源
《Infection and immunity》 |1994年第6期|共7页
作者
T Hensler; B K?nig; G Prévost; Y Piémont; M K?ller; W K?nig;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类医学免疫学;
关键词

相似文献

外文文献
中文文献
专利

1. Differential immunostimulating effect of Granulocyte-macrophage colony-stimulating factor (GM-CSF), Granulocyte colony-stimulating factor (G-CSF) and Interferon gamma (IFNgamma) after severe trauma. [J] . Lendemans S, Kreuzfelder E, Waydhas C, Inflammation research: Official journal of the European Histamine Research Society . 2007,第1期

机译：严重创伤后粒细胞-巨噬细胞集落刺激因子（GM-CSF），粒细胞集落刺激因子（G-CSF）和干扰素γ（IFNγ）的差异免疫刺激作用。
2. Differential immunostimulating effect of Granulocyte-macrophage colony-stimulating factor (GM-CSF), Granulocyte colony-stimulating factor (G-CSF) and Interferon γ (IFNγ) after severe trauma [J] . S. Lendemans, E. Kreuzfelder, C. Waydhas, Inflammation Research . 2007,第1期

机译：严重创伤后粒细胞巨噬细胞集落刺激因子（GM-CSF），粒细胞集落刺激因子（G-CSF）和干扰素γ（IFNγ）的差异免疫刺激作用
3. Cross-linking of human FcgammaRIIIb induces the production of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor by polymorphonuclear neutrophils. [J] . Durand V, Renaudineau Y, Pers JO, The Journal of Immunology: Official Journal of the American Association of Immunologists . 2001,第7期

机译：人FcgammaRIIIb的交联诱导多形核中性粒细胞产生粒细胞集落刺激因子和粒细胞巨噬细胞集落刺激因子。
4. Granulocyte-macrophage colony-stimulating factor-activated signaling pathways in human neutrophils. The mechanism of activation of phosphatidylinositol 3-kinase [D] . Al-Shami, Amin 1998

机译：人类嗜中性粒细胞中的粒细胞-巨噬细胞集落刺激因子激活的信号通路。磷脂酰肌醇3-激酶的激活机制
5. Leukotriene B4 generation and DNA fragmentation induced by leukocidin from Staphylococcus aureus: protective role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF for human neutrophils. [O] . T Hensler, B König, G Prévost, 1994

机译：金黄色葡萄球菌中白三烯生成的白三烯B4生成和DNA片段化：粒细胞巨噬细胞集落刺激因子（GM-CSF）和G-CSF对人中性粒细胞的保护作用。
6. Granulocyte-macrophage colony-stimulating factor (GM-CSF) but not granulocyte colony-stimulating factor (G-CSF) induces plasma membrane expression of proteinase 3 (PR3) on neutrophils in vitro [O] . Hellmich, B, Csernok, E, Trabandt, A, 2000

机译：粒细胞巨噬细胞集落刺激因子（GM-CSF）而非粒细胞集落刺激因子（G-CSF）诱导中性粒细胞体外蛋白酶3（PR3）的质膜表达
7. Interdependence of the Radioprotective Effects of Human Recombinant Interleukin 1alpha, Tumor Necrosis Factor alpha, Granulocyte Colony-Stimulating Factor, and Murine Recombinant Granulocyte-Macrophage Colony-Stimulating Factor [R] . Neta, R., Oppenheim, J. J., Douches, S. D. 1988

机译：人重组白细胞介素1α，肿瘤坏死因子α，粒细胞集落刺激因子和小鼠重组粒细胞 - 巨噬细胞集落刺激因子的辐射保护作用的相互依赖性

Leukotriene B4 generation and DNA fragmentation induced by leukocidin from Staphylococcus aureus: protective role of granulocyte-macrophage colony-stimulating factor (GM-CSF) and G-CSF for human neutrophils.

摘要

著录项

相似文献

相关主题

期刊订阅