• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Induction of interleukin-1 from murine peritoneal macrophages by Pseudomonas aeruginosa exotoxin A.
【24h】

Induction of interleukin-1 from murine peritoneal macrophages by Pseudomonas aeruginosa exotoxin A.

机译：铜绿假单胞菌外毒素A从鼠腹膜巨噬细胞诱导白细胞介素-1。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pseudomonas exotoxin A, an ADP-ribosylating toxin produced by Pseudomonas aeruginosa, has been shown to stimulate the proliferation of murine thymocytes, which requires the participation of accessory cells. This requirement for accessory cells can be replaced by supernatant from adherent peritoneal exudate cells that have been stimulated with exotoxin A. Antibody to exotoxin A inhibits the induction of the thymocyte mitogenic activity from adherent peritoneal macrophages. However, antibody to exotoxin A had no effect on the thymocyte proliferation if the antibody was added to supernatant which contained thymocyte mitogenic activity. The thymocyte mitogenic activity was associated with a protein or protein complex with a molecular mass of greater than 10,000 daltons. D10 bioassays indicated the presence of interleukin-1 (IL-1) in the supernatant. Antibody to IL-1 inhibited the ability of supernatant to induce thymocytes to proliferate. Therefore, these data suggest that Pseudomonas exotoxin A can stimulate the production of IL-1 from adherent peritoneal cells, which induces murine thymocytes to proliferate.
机译：铜绿假单胞菌产生的ADP-核糖基化毒素假单胞菌外毒素A已显示出刺激鼠胸腺细胞的增殖，这需要辅助细胞的参与。对辅助细胞的这种要求可以由已经用外毒素A刺激的粘附性腹膜分泌液细胞的上清液代替。外毒素A的抗体抑制了粘附性腹膜巨噬细胞对胸腺细胞有丝分裂活性的诱导。但是，如果将抗体加入到含有胸腺细胞有丝分裂活性的上清液中，则外毒素A抗体对胸腺细胞增殖没有影响。胸腺细胞的促有丝分裂活性与分子量大于10,000道尔顿的蛋白质或蛋白质复合物有关。 D10生物测定表明上清液中存在白介素-1（IL-1）。 IL-1抗体抑制上清液诱导胸腺细胞增殖的能力。因此，这些数据表明假单胞菌外毒素A可以刺激粘附的腹膜细胞产生IL-1，从而诱导鼠胸腺细胞增殖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号