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首页> 外文期刊>Infection and immunity >Induction of Epithelial Cell Death Including Apoptosis by Enteropathogenic Escherichia coli Expressing Bundle-Forming Pili
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Induction of Epithelial Cell Death Including Apoptosis by Enteropathogenic Escherichia coli Expressing Bundle-Forming Pili

机译：表达集束形成菌毛的肠致病性大肠杆菌诱导上皮细胞死亡（包括凋亡）

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摘要

Infection with enteropathogenic Escherichia coli (EPEC) is a major cause of severe infantile diarrhea, particularly in parts of the developing world. The bundle-forming pilus (BFP) of EPEC is an established virulence factor encoded on the EPEC adherence factor plasmid (EAF) and has been implicated in both localized adherence to host cells and bacterial autoaggregation. We investigated the role of BFP in the ability of EPEC binding to kill host epithelial cells. BFP-expressing strains killed all three cell lines tested, comprising HEp-2 (laryngeal), HeLa (cervical), and Caco-2 (colonic) cells. Analysis of phosphatidylserine expression, internucleosomal cleavage of host cell DNA, and morphological changes detected by electron microscopy indicated evidence of apoptosis. The extent of cell death was significantly greater for BFP-expressing strains, including E2348/69, a wild-type clinical isolate, as well as for a laboratory strain, HB101, transformed with a bfp-carrying plasmid. Strains which did not express BFP induced significantly less cell death, including a bfpA disruptional mutant of E2348/69, EAF plasmid-cured E2348/69, HB101, and HB101 complemented with the locus of enterocyte effacement pathogenicity island. These results indicate a direct correlation between BFP expression and induction of cell death, including apoptosis, an event which may involve the targeting of host cell membrane phosphatidylethanolamine.
机译：肠致病性大肠埃希菌（EPEC）感染是婴儿严重腹泻的主要原因，尤其是在发展中国家的部分地区。 EPEC的成束菌毛（BFP）是一种在EPEC粘附因子质粒（EAF）上编码的确定的毒力因子，并且与宿主细胞的局部粘附和细菌自动聚集有关。我们调查了BFP在EPEC结合杀死宿主上皮细胞的能力中的作用。表达BFP的菌株杀死了测试的所有三个细胞系，包括HEp-2（喉），HeLa（子宫颈）和Caco-2（结肠）细胞。通过电子显微镜检测到的磷脂酰丝氨酸表达，宿主细胞DNA的核小体切割以及形态学变化的分析表明存在凋亡的迹象。对于表达BFP的菌株，包括野生型临床分离株E2348 / 69，以及用携带 bfp 的质粒转化的实验室菌株HB101，细胞死亡的程度明显更高。 。不表达BFP的菌株诱导的细胞死亡显着减少，包括E2348 / 69的 bfpA 破坏突变体，EAF质粒固化的E2348 / 69，HB101和HB101，并补充了肠上皮细胞致病性病原体。 。这些结果表明BFP表达与细胞死亡的诱导，包括细胞凋亡，之间的直接关系，这一事件可能涉及靶向宿主细胞膜磷脂酰乙醇胺。

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