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首页> 外文期刊>Infection and immunity >Carrageenan Primes Leukocytes To Enhance Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Production
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Carrageenan Primes Leukocytes To Enhance Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Production

机译：角叉菜胶引发白细胞增强脂多糖诱导的肿瘤坏死因子α的产生

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摘要

We have previously reported that pretreatment with carrageenan (CAR) enhances lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α) production in and lethality for mice. Whole blood cultured in vitro was used to show that CAR pretreatment results in about a 200-fold increase in LPS-induced TNF-α production. CAR by itself did not induce TNF-α production. However, CAR-treated cultured medium sensitized whole blood to make more LPS-induced TNF than did saline-treated cultured medium in vitro. It was also demonstrated that CAR pretreatment increases TNF-α mRNA levels of both blood cells and peritoneal exudate cells, but not of bone marrow cells. Immunoelectron microscopic analysis revealed that polymorphonuclear leukocytes and macrophages are TNF-α-producing cells in CAR-treated mice. In CAR-treated mice, TNF-α was seen early after LPS injection in leukocytes in hepatic sinusoids and on the surfaces of endothelial cells. TNF-α was also detected late after LPS injection in hepatocytes which become edematous. These results suggest that CAR primes leukocytes to produce TNF-α in response to LPS and that they play an important role in the pathogenesis of liver injury.
机译：我们以前曾报道过用角叉菜胶（CAR）进行预处理可增强脂多糖（LPS）诱导的小鼠肿瘤坏死因子α（TNF-α）的产生和致死性。体外培养的全血用于显示CAR预处理导致LPS诱导的TNF-α产量增加约200倍。 CAR本身不会诱导TNF-α的产生。然而，与盐水处理的培养基相比，CAR处理的培养基对全血的敏感性更高，从而使LPS诱导的TNF含量更高。还证明了CAR预处理可增加血细胞和腹膜渗出细胞的TNF-αmRNA水平，但不增加骨髓细胞的TNF-αmRNA水平。免疫电子显微镜分析显示，在CAR处理的小鼠中，多形核白细胞和巨噬细胞是产生TNF-α的细胞。在接受CAR治疗的小鼠中，LPS注射后早期在肝窦中的白细胞和内皮细胞表面上可见TNF-α。 LPS注射后的肝细胞中，水肿后也检测到了TNF-α。这些结果表明，CAR可引发白细胞产生对LPS的TNF-α，并且它们在肝损伤的发病机理中起重要作用。

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