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Ruminant Gastrointestinal Cell Proliferation and Clearance of Escherichia coli O157:H7

机译：反刍型胃肠细胞的增殖和大肠杆菌O157：H7的清除

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Human infections with Escherichia coli O157:H7 cause hemorrhagic colitis that can progress to a life-threatening sequelae. The most common mode of disease transmission is ingestion of contaminated bovine food products, and it is well established thatE. coli O157:H7 is a transient member of the bovine microbiota. However, the conditions that induce acquisition and subsequent clearance of this bacterium from the ruminant gastrointestinal tract (GIT) are not understood. Evidence that the rates of epithelial cell proliferation in the lower GIT of cattle are associated with the duration animals remained E. coliO157:H7 culture positive is presented. Cattle with slower rates of intestinal cell proliferation in the cecum and the distal colon were culture positive significantly longer than cohort cattle with faster cell proliferation rates. Cell death rates (apoptotic indices) between the short- and long-term culture-positive animals were not different. Typical grain-based finishing diets and forage-based growing diets did not effect GIT cell proliferation or the duration animals remained E. coli O157:H7 culture positive. To identify a dietary intervention that would effect GIT cell proliferation, we used sheep as a model ruminant. A fasting-refeeding regime that increased the rate of GIT cell proliferation was developed. The fasting-refeeding protocol was used in cattle to test the hypothesis that feeding interventions that increase the rate of GIT cell proliferation induce the clearance of E. coli O157:H7 from the bovine GIT.
机译：人类感染O157：H7大肠杆菌会导致出血性结肠炎，并可能发展为威胁生命的后遗症。疾病传播的最常见方式是摄入受污染的牛食品，并且众所周知E。 O157：H7大肠杆菌是牛微生物群的瞬时成员。但是，导致该细菌从反刍动物胃肠道（GIT）的获取和随后清除的条件尚不清楚。证据表明牛GIT较低的上皮细胞增殖速率与动物大肠杆菌E157：H7培养阳性的持续时间有关。盲肠和远端结肠中肠道细胞增殖速度较慢的牛比具有较高细胞增殖速度的同类牛的培养阳性时间长得多。短期和长期培养阳性动物之间的细胞死亡率（凋亡指数）没有差异。典型的以谷物为基础的日粮和以饲料为基础的生长日粮不会影响GIT细胞的增殖，或者动物在大肠杆菌O157：H7培养中保持阳性的持续时间。为了确定将影响GIT细胞增殖的饮食干预措施，我们使用绵羊作为反刍动物模型。建立了一种禁食-进食机制，可以提高GIT细胞的增殖速率。在牛中使用禁食-再喂食方案来检验以下假设：提高GIT细胞增殖速率的喂食干预措施可诱导牛GIT清除大肠杆菌O157：H7。

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