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首页> 外文期刊>Infection and immunity >Antigenic drift of Haemophilus influenzae in patients with chronic obstructive pulmonary disease.
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Antigenic drift of Haemophilus influenzae in patients with chronic obstructive pulmonary disease.

机译：慢性阻塞性肺疾病患者流感嗜血杆菌的抗原漂移。

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摘要

Differences in the major outer membrane protein b,c (molecular weight, 39,000 to 41,000) of related Haemophilus influenzae strains isolated from the sputum of patients with chronic obstructive pulmonary disease were analyzed biochemically and immunologically. Protein b,c was isolated from a total of six related H. influenzae strains from two chronic obstructive pulmonary disease patients. After CNBr digestion of the proteins, the differences in size appeared in the larger of the two fragments. Trypsin and chymotrypsin digests of proteins from related H. influenzae strains showed that proteins differed by only a few peptides or were very similar, in contrast to the peptide maps of proteins from nonrelated strains. Peptide analysis of b,c proteins from related H. influenzae strains by high-performance liquid chromatography after Staphylococcus aureus V8 protease digestion and amino acid analysis of corresponding fractions revealed highly comparable patterns, indicating only minor differences in the amino acid sequences of these proteins. Immunization of rabbits with intact bacteria of four related H. influenzae strains resulted in a strong anti-protein b,c antibody response in all animals. In three of four rabbits, antibodies specific for the b,c protein of the strain used for immunization were elicited, indicating that the changed proteins contained specific immunodominant epitopes. Anti-protein b,c antibodies promoted strain-specific, complement-dependent, bactericidal activity. From these results, we conclude that H. influenzae shows antigenic drift in immunodominant epitopes, caused by small changes in amino acid composition of the b,c protein. Antibodies to these epitopes promote complement-dependent bactericidal activity.
机译：从慢性阻塞性肺疾病患者的痰液中分离出的相关流感嗜血杆菌菌株的主要外膜蛋白b，c（分子量，39,000至41,000）之间的差异已通过生化和免疫学方法进行了分析。从两名慢性阻塞性肺疾病患者的六种相关流感嗜血杆菌菌株中分离出蛋白b，c。 CNBr消化蛋白质后，两个片段中较大的一个出现了大小差异。来自相关流感嗜血杆菌菌株的蛋白质的胰蛋白酶和胰凝乳蛋白酶消化表明，与不相关菌株的蛋白质的肽图相比，该蛋白质仅相差几个肽或非常相似。金黄色葡萄球菌V8蛋白酶消化后，通过高效液相色谱对相关流感嗜血杆菌菌株b，c蛋白进行肽分析，并对相应馏分进行氨基酸分析，结果显示出高度可比的模式，表明这些蛋白的氨基酸序列仅有微小差异。用四种相关的流感嗜血杆菌菌株的完整细菌免疫兔后，在所有动物中均产生了强烈的抗蛋白b，c抗体反应。在四只兔子中的三只中，产生了对用于免疫的菌株的b，c蛋白具有特异性的抗体，表明所改变的蛋白包含特定的免疫优势表位。抗蛋白b，c抗体可促进菌株特异性，补体依赖性杀菌活性。从这些结果，我们得出结论，流感嗜血杆菌显示免疫优势表位中的抗原漂移，这是由b，c蛋白氨基酸组成的微小变化引起的。这些表位的抗体促进补体依赖性杀菌活性。

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