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首页> 外文期刊>Infection and immunity >The Stress-Responsive dgk Gene from Streptococcus mutans Encodes a Putative Undecaprenol Kinase Activity
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The Stress-Responsive dgk Gene from Streptococcus mutans Encodes a Putative Undecaprenol Kinase Activity

机译：来自变形链球菌的应激反应dgk基因编码假定的十一碳烯醇激酶活性。

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摘要

We analyzed a previously constructed stress-sensitive Streptococcus mutans mutant Tn-1 strain resulting from disruption by transposon Tn916 of a gene encoding a protein exhibiting amino acid sequence similarity to the Escherichia coli diacylglycerol kinase. It was confirmed that the mutation led to significantly reduced lipid kinase activity, while expression of the intact gene on a plasmid restored both kinase activity and the wild-type phenotype. Further analysis revealed that the product of the dgk gene in S. mutans predominantly recognizes a lipid substrate other than diacylglycerol, most likely undecaprenol, as demonstrated by its efficient phosphorylation and the resistance of the product of the reaction to saponification. The physiological role of the product of the dgk gene as a putative undecaprenol kinase was further supported by a significantly higher sensitivity of the mutant to bacitracin compared with that of the parental strain.
机译：我们分析了先前构建的应激敏感的变形链球菌（Stremococcus mutans）突变体Tn-1菌株，该菌株是由转座子Tn 916 破坏的，该基因编码的氨基酸序列与<大肠埃希氏菌二酰基甘油激酶。证实了该突变导致脂质激酶活性显着降低，而质粒上完整基因的表达恢复了激酶活性和野生型表型。进一步的分析表明， dgk 基因的产物在 S中。变异蛋白主要识别除二酰基甘油以外的脂质底物，最有可能是十一碳烯醇，这通过其有效的磷酸化作用和反应产物对皂化的抵抗力来证明。突变体对杆菌肽的敏感性明显高于亲本菌株，进一步证明了 dgk 基因产物作为假定的十一碳烯醇激酶的生理作用。

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