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首页> 外文期刊>Infection and immunity >Binding of the two components of C2 toxin to epithelial cells and brush borders of mouse intestine.
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Binding of the two components of C2 toxin to epithelial cells and brush borders of mouse intestine.

机译：C2毒素的两种成分与小鼠肠上皮细胞和刷状缘的结合。

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摘要

C2 toxin elaborated by Clostridium botulinum types C and D is composed of two nonlinked protein components and has enterotoxic activity, for which the cooperation of these two components is necessary. In the present study, the binding of components I and II, the two components of C2 toxin, to isolated epithelial cells and brush borders of mouse intestine was examined. Immunofluorescence studies showed that component II, either trypsinized (T-II) or untrypsinized (UT-II), bound to the cells and the brush borders of mouse intestine, whereas component I alone did not. The binding of I was observed only when the cells and the brush borders were reacted with T-II, but not when they were reacted with UT-II. These results are consistent with the fact that the biological activities of C2 toxin are elicited by the combination of I and T-II, but not of I and UT-II. The in vitro binding of I and II to isolated brush borders of mouse intestinal cells also showed similar binding characteristics. The binding of I and II to brush borders was rapid and not temperature dependent. Ultracentrifugal analysis revealed that both I and T-II bound to microvillous membranes of the intestinal cells. The data from the present study indicate that the enterotoxic activity of C2 toxin is initiated by the binding of T-II to the microvillous membrane of intestinal cells followed by that of I, for which the site of the cell membrane is induced by the binding of T-II, but not of UT-II.
机译：由肉毒梭菌C型和D型精心制作的C2毒素由两个未连接的蛋白质成分组成，并具有肠毒性活性，为此，这两个成分必须协同作用。在本研究中，检查了组分I和II（C2毒素的两个组分）与小鼠肠的分离上皮细胞和刷状缘的结合。免疫荧光研究表明，用胰蛋白酶处理的（T-II）或未用胰蛋白酶处理的（UT-II）的成分II与小鼠肠道的细胞和刷状缘结合，而单独的成分I则没有。仅当细胞和刷状缘与T-II反应时才观察到I的结合，而当它们与UT-II反应时未观察到I的结合。这些结果与C2毒素的生物活性是由I和T-II的组合而不是I和UT-II的组合引起的事实相一致。 I和II与小鼠肠细胞的分离刷状边界的体外结合也显示出相似的结合特性。 I和II与刷边界的结合是快速的，并且与温度无关。超速离心分析显示，I和T-II均与肠细胞的微毛膜结合。来自本研究的数据表明，C2毒素的肠毒性活性是由T-II与肠细胞的微毛膜结合，然后由I结合而引起的，对于I2，肠膜的结合是诱导细胞膜的部位T-II，但不包括UT-II。

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