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首页> 外文期刊>Infection and immunity >Inhibition of human neutrophil chemiluminescence by plasmid-mediated outer membrane proteins of Yersinia enterocolitica.
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Inhibition of human neutrophil chemiluminescence by plasmid-mediated outer membrane proteins of Yersinia enterocolitica.

机译：质粒介导的小肠结肠炎耶尔森氏菌外膜蛋白抑制人嗜中性粒细胞的化学发光。

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Recent studies have shown that the cell surface properties of Yersinia enterocolitica are altered by the presence of the virulence plasmid, which mediates temperature-inducible outer membrane proteins (OMP). We investigated the interaction of Y. enterocolitica with human polymorphonuclear leukocytes by monitoring luminol-enhanced chemiluminescence (CL) responses. A plasmid-bearing strain grown at 37 degrees C induced four- to sixfold less CL than did the same strain grown at 25 degrees C or a plasmidless, isogenic strain grown at either temperature. Inhibition of CL responses by whole cells was related to plasmid-mediated expression of OMP. The OMP alone could inhibit the CL response of polymorphonuclear leukocytes stimulated by either opsonized zymosan or whole cells of Y. enterocolitica. Pronase treatment of whole cells, which removed the plasmid-mediated OMP, resulted in partial but significant elimination of CL inhibition by whole cells and by OMP derived from them. Incubation with Y. enterocolitica for 60 min did not affect the viability of polymorphonuclear leukocytes. Our results suggest that the interaction of Y. enterocolitica with human polymorphonuclear leukocytes is directly affected by the plasmid-mediated OMP.
机译：最近的研究表明，小肠结肠炎耶尔森氏菌的细胞表面特性会通过介导温度诱导性外膜蛋白（OMP）的毒性质粒的存在而改变。我们通过监测鲁米诺增强的化学发光（CL）反应，调查了小肠结肠炎耶尔森菌与人多形核白细胞的相互作用。与在25摄氏度下生长的相同菌株或在任一温度下生长的无质粒等基因菌株相比，在37摄氏度下生长的带有质粒的菌株诱导的CL减少四到六倍。全细胞对CL应答的抑制与质粒介导的OMP表达有关。单独的OMP可以抑制调理的酵母聚糖或小肠结肠炎耶尔森氏菌全细胞刺激的多形核白细胞的CL反应。对全细胞进行链霉蛋白酶处理，去除了质粒介导的OMP，导致全细胞及其衍生的OMP对CL抑制的部分但显着消除。与小肠结肠炎耶尔森氏菌孵育60分钟不会影响多形核白细胞的生存能力。我们的结果表明，小肠结肠炎耶尔森菌与人多形核白细胞的相互作用直接受到质粒介导的OMP的影响。

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