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首页> 外文期刊>Infection and immunity >Detection of antibodies against lipopolysaccharides of Escherichia coli and Salmonella R and S strains by immunoblotting.
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Detection of antibodies against lipopolysaccharides of Escherichia coli and Salmonella R and S strains by immunoblotting.

机译：通过免疫印迹检测针对大肠杆菌和沙门氏菌R和S菌株的脂多糖的抗体。

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摘要

Antisera raised against several smooth and rough strains of Escherichia coli and Salmonella typhimurium were tested against lipopolysaccharides (LPS) of homologous and heterologous strains. The LPS were separated by sodium dodecyl sulfate-gel electrophoresis, transferred to nitrocellulose paper, and overlaid with antisera. The results showed that antisera raised against smooth strains reacted with high- as well as low-molecular-weight bands of their corresponding LPS and showed very few cross-reactions. Anti-E. coli J5 antiserum cross-reacted with few strains in the core region. But, anti-S. typhimurium Ra antiserum cross-reacted with many more strains. When these sera were absorbed with either the homologous- or a heterologous-positive strain, reactions were abolished. It appears that reactions of anti-E. coli J5 antiserum and anti-S. typhimurium Ra antiserum with homologous and heterologous strains were not due to the same antibody. This immunoblotting technique proved to be a useful method to distinguish different antibodies in antiserum raised against LPS of gram-negative bacteria.
机译：测试了针对大肠杆菌和鼠伤寒沙门氏菌的几种平滑和粗糙菌株产生的抗血清针对同源和异源菌株的脂多糖（LPS）。通过十二烷基硫酸钠-凝胶电泳分离LPS，转移到硝酸纤维素纸上，并用抗血清覆盖。结果表明，针对平滑菌株产生的抗血清与其相应的LPS的高分子量和低分子量谱带发生反应，并且几乎没有交叉反应。反E。大肠杆菌J5抗血清与核心区域中的少数菌株交叉反应。但是，反S。鼠伤寒Ra抗血清与许多其他菌株交叉反应。当这些血清被同源或异源阳性菌株吸收时，反应被消除。看来抗E反应。大肠杆菌J5抗血清和抗S。鼠伤寒沙门氏菌Ra抗血清具有同源和异源菌株不是由于相同的抗体。这种免疫印迹技术被证明是区分抗革兰氏阴性菌LPS产生的抗血清中不同抗体的有用方法。

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