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首页> 外文期刊>Infection and immunity >Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars.
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Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars.

机译：15个沙眼衣原体血清型主要外膜蛋白的四个可变域的核苷酸序列和推导的氨基酸序列。

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The amino acid sequences of major outer membrane proteins (MOMPs) from Chlamydia trachomatis serovars A, B, C, L1, and L2 are predominantly conserved but have four variable domains (VDs) in which major neutralizing and serotyping antigenic determinants are located. Because these MOMP VDs are primarily responsible for antigenic differences between serovars and are associated with important immunological and biological properties, we undertook studies focused on defining these sequences within the MOMPs of all 15 C. trachomatis serovars. We used oligonucleotide primer extension sequencing of MOMP mRNA to determine the nucleotide and deduced amino acid sequences of the four MOMP VDs of the 15 C. trachomatis serovars. Comparative amino acid sequence homologies of all four domains separated the serovars into three groups: group 1, serovars B, Ba, D, E, L1, and L2; group 2, serovars G and F; and group 3, serovars A, C, H, I, J, K, and L3. Hydrophilicity and charge values for each domain were determined. The MOMP VDs of given serovars with the greatest total hydrophilicity and charge values were found to be the location of antigenic determinants recognized by MOMP-specific monoclonal antibodies. These findings should be useful for predicting MOMP antigenic determinants and testing the antigenic properties of these VDs by using synthetic peptides corresponding to each MOMP VD. The potential usefulness of the VD sequence information is discussed in relation to the development of defined synthetic peptides and oligonucleotides that may be used to develop new serological and diagnostic assays for C. trachomatis infections.
机译：沙眼衣原体血清型A，B，C，L1和L2的主要外膜蛋白（MOMP）的氨基酸序列主要是保守的，但具有四个可变域（VD），主要的中和和血清型抗原决定簇位于其中。因为这些MOMP VD主要负责血清型之间的抗原差异，并且与重要的免疫学和生物学特性有关，所以我们进行了研究，重点是在所有15个沙眼衣原体血清型MOMP中定义这些序列。我们使用MOMP mRNA的寡核苷酸引物延伸测序来确定15个沙眼衣原体血清型的四个MOMP VD的核苷酸和推导的氨基酸序列。所有四个结构域的比较氨基酸序列同源性将血清型分为三组：第一组，血清型B，Ba，D，E，L1和L2;血清型B，Ba，D，E，L1和L2。组2，血清型G和F;第3组，血清型A，C，H，I，J，K和L3。确定每个区域的亲水性和电荷值。发现具有最大的总亲水性和电荷值的给定血清型的MOMP VD是被MOMP特异性单克隆抗体识别的抗原决定簇的位置。这些发现对于预测MOMP抗原决定簇和通过使用对应于每个MOMP VD的合成肽测试这些VD的抗原特性应该是有用的。关于确定的合成肽和寡核苷酸的开发，讨论了VD序列信息的潜在有用性，这些合成的肽和寡核苷酸可用于开发沙眼衣原体感染的新血清学和诊断检测方法。

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