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首页> 外文期刊>Infection and immunity >Neisseria lactamica and Neisseria meningitidis share lipooligosaccharide epitopes but lack common capsular and class 1, 2, and 3 protein epitopes.
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Neisseria lactamica and Neisseria meningitidis share lipooligosaccharide epitopes but lack common capsular and class 1, 2, and 3 protein epitopes.

机译：乳酸奈瑟菌和脑膜炎奈瑟菌共享脂寡糖表位，但缺乏常见的荚膜和1、2和3类蛋白表位。

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Neisseria lactamica, a common human pharyngeal commensal, contributes to acquired immunity to Neisseria meningitidis. To define the surface antigens shared between these two species, we used monoclonal antibodies (MAbs) to study 35 N. lactamica strains isolated in various parts of the world for cross-reactivity with meningococcal capsules, outer membrane proteins, and lipooligosaccharides (LOS). No N. lactamica strain reacted significantly with MAbs specific for capsular group A, B, C, Y, or W, and we were unable to extract capsular polysaccharide from them. Only 2 of 33 strains reacted weakly with MAbs against class 2 serotype proteins P2b and P2c. None reacted with MAbs specific for meningococcal class 1 protein P1.2 or P1.16 or class 2/3 serotype protein P2a or P15. Most N. lactamica strains (30 of 35) bound one or more of seven LOS-specific MAbs. Two LOS epitopes, defined by MAbs O6B4 and 3F11, that are commonly found on pathogenic Neisseria species were found on 25 of 35 N. lactamica. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting showed that the LOS of N. lactamica are composed of multiple components that are physically and antigenically similar to the LOS of pathogenic Neisseria species. Among four other commensal neisserial species, only Neisseria cinerea shared LOS epitopes defined by MAbs O6B4 and 3F11. Previous studies have shown that pharyngeal colonization with N. lactamica induces bactericidal antibodies against the meningococcus. We postulate that shared N. lactamica and meningococcal LOS epitopes may play an important role in the development of natural immunity to the meningococcus.
机译：乳酸奈瑟氏菌，一种常见的人类咽膜，有助于获得针对脑膜炎奈瑟菌的免疫力。为了定义这两个物种之间共享的表面抗原，我们使用单克隆抗体（MAbs）研究了在世界各地分离出的35株N. lactamica菌株与脑膜炎球菌胶囊，外膜蛋白和脂寡糖（LOS）的交叉反应性。没有乳酸杆菌菌株与针对荚膜组A，B，C，Y或W的单克隆抗体发生显着反应，因此我们无法从中提取荚膜多糖。 33个菌株中只有2个与MAb对2类血清型蛋白P2b和P2c的反应较弱。没有人与特异于脑膜炎球菌1类蛋白P1.2或P1.16或2/3类血清型蛋白P2a或P15的单克隆抗体反应。大多数乳酸杆菌菌株（35个中的30个）结合七个或多个LOS特异性MAb中的一个或多个。在35个N. lactamica中发现了两个LOS抗原决定簇，由MAbs O6B4和3F11定义，通常在致病性奈瑟氏菌属物种上发现。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹分析表明，乳酸奈瑟菌的LOS由多种成分组成，这些成分在物理上和抗原上类似于致病性奈瑟氏菌属的LOS。在其他四个共生奈瑟菌属物种中，只有灰黄奈瑟氏菌共享由单克隆抗体O6B4和3F11定义的LOS表位。先前的研究表明，用乳杆菌猪笼草定殖会诱导针对脑膜炎球菌的杀菌抗体。我们推测，共有的乳球菌和脑膜炎球菌LOS表位可能在对脑膜炎球菌的天然免疫力发展中起重要作用。

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