...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Dynamic analysis of splenic Th1 and Th2 lymphocyte functions in mice infected with Schistosoma japonicum.
【24h】

Dynamic analysis of splenic Th1 and Th2 lymphocyte functions in mice infected with Schistosoma japonicum.

机译：日本血吸虫感染小鼠脾脏Th1和Th2淋巴细胞功能的动态分析。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent studies indicate that egg granuloma formation in murine Schistosoma mansoni infection is associated with Th2-mediated immune responses. The present study was designed to analyze dynamically the Th1 and Th2 responses in S. japonicum-infected animals and compare them with the results seen with S. mansoni. C3H mice were infected with 10 to 20 cercariae of S. japonicum and sacrificed 3 to 22 weeks later. Spleen cells were stimulated with parasite antigens (egg and adult worm) or the mitogen concanavalin A. Interleukin-2 (IL-2), IL-4, IL-5, and gamma interferon (IFN-gamma) levels were measured in the culture supernatants by enzyme-linked immunosorbent assay (ELISA) or bioassays. Additionally, cytokine-producing cells were enumerated by ELISPOT. The results show that Th2 cytokine production, characterized by IL-4 and IL-5, represents the major response in the first month after egg laying begins, while the Th1 functions of IFN-gamma and IL-2 production are greatly depressed. However, by 22 weeks Th2 responses have diminished and IFN-gamma production in response to concanavalin A is apparent. IL-2 responses are minimal at all times. In vitro depletion of T-cell subsets indicates that CD4+ cells are the major subset responsible for production of IL-5 at 7 weeks of infection. These findings suggest that, as in the case of S. mansoni infection, S. japonicum-induced immunopathology is temporally associated with the host Th2 response, although other experiments indicate that IFN-gamma is also involved.
机译：最近的研究表明，在曼氏血吸虫鼠感染中卵肉芽肿的形成与Th2介导的免疫反应有关。本研究旨在动态分析日本链球菌感染动物中的Th1和Th2反应，并将其与曼氏链球菌观察到的结果进行比较。 C3H小鼠感染了10至20个日本血吸虫尾，并在3至22周后处死。用寄生虫抗原（例如卵和成虫）或有丝分裂伴刀豆球蛋白A刺激脾细胞。在培养物中测量白介素2（IL-2），IL-4，IL-5和γ干扰素（IFN-γ）的水平酶联免疫吸附测定（ELISA）或生物测定获得上清液。另外，通过ELISPOT列举产生细胞因子的细胞。结果表明，以IL-4和IL-5为特征的Th2细胞因子的产生代表着卵开始后第一个月的主要反应，而IFN-γ和IL-2产生的Th1功能却大大降低。但是，到22周时，Th2反应减弱，对伴刀豆球蛋白A的IFN-γ产生也很明显。 IL-2反应始终是最小的。 T细胞亚群的体外耗竭表明CD4 +细胞是感染7周时产生IL-5的主要亚群。这些发现表明，与曼氏链球菌感染的情况一样，日本血吸虫诱导的免疫病理学在时间上与宿主Th2应答相关，尽管其他实验表明也涉及IFN-γ。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号