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首页> 外文期刊>Infection and immunity >Adherence of oral streptococci to salivary glycoproteins.
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Adherence of oral streptococci to salivary glycoproteins.

机译：口腔链球菌对唾液糖蛋白的粘附。

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We used an overlay method to study the ability of human salivary glycoproteins to serve as receptors for several strains of streptococci that colonize the oral cavity. Parotid and submandibular-sublingual salivas were collected as ductal secretions, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes. The resulting blots were overlaid with [35S]methionine-labeled bacteria, and salivary components to which the bacteria bound were detected by autoradiography. Potential glycoprotein receptors were identified for 8 of the 16 strains tested. In three cases (Streptococcus sanguis 72-40 and 804 and Streptococcus sobrinus OMZ176), highly specific interactions with a single salivary component were detected. Removal of sialic acid residues from the low-molecular-weight salivary mucin prevented adherence of one of these strains (S. sanguis 72-40), suggesting that this saccharide either mediates binding or is a critical component of the receptor site. In the remaining five strains (Streptococcus gordonii G9B and 10558, S. sanguis 10556, and Streptococcus oralis 10557 and 72-41), interactions with multiple salivary components, including the low-molecular-weight salivary mucin, highly glycosylated proline-rich glycoproteins, and alpha-amylase, were detected. These results suggest that some oral streptococci can bind specifically to certain of the salivary glycoproteins. The interactions identified may play an important role in governing bacterial adherence and clearance within the oral cavity.
机译：我们使用了一种覆盖方法来研究人类唾液糖蛋白作为几种定植在口腔中的链球菌菌株的受体的能力。收集腮腺和颌下舌下唾液作为导管分泌物，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，并转移至硝酸纤维素膜上。所得的印迹被[35S]蛋氨酸标记的细菌覆盖，并且通过放射自显影检测与细菌结合的唾液成分。在测试的16个菌株中，有8个被鉴定出潜在的糖蛋白受体。在三例情况下（血链球菌72-40和804和sobrinus链霉菌OMZ176），检测到了与单个唾液成分的高度特异性相互作用。从低分子量唾液粘蛋白​​中除去唾液酸残基阻止了这些菌株之一的粘附（S.sanguis 72-40），表明该糖介导结合或是受体位点的关键成分。在其余五种菌株（戈登链球菌G9B和10558，桑氏链球菌10556和口腔链球菌10557和72-41）中，它们与多种唾液成分相互作用，包括低分子量唾液粘蛋白​​，高度糖基化脯氨酸丰富的糖蛋白，和α-淀粉酶。这些结果表明，某些口服链球菌可以与某些唾液糖蛋白特异性结合。鉴定出的相互作用可能在控制细菌在口腔内的粘附和清除中起重要作用。

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