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Effect of environmental conditions on production of toxic shock syndrome toxin 1 by Staphylococcus aureus.

机译:环境条件对金黄色葡萄球菌中毒休克综合征毒素1产生的影响。

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The kinetics of toxic shock syndrome toxin 1 (TSST-1) production by Staphylococcus aureus was studied in a fermentor in which aeration rate, atmospheric composition, pH, and temperature were controlled. The toxin was synthesized at a maximal rate during the exponential phase. High bacterial populations were not necessarily accompanied by high TSST-1 yields. Aerobiosis increased TSST-1 production, but excessive aeration had an adverse effect. Addition of CO2 enhanced TSST-1 yield by increasing toxin production rate and efficiency. Cultures with no pH control made more TSST-1 than those maintained at pH 5.5 to 7.5. Maximum TSST-1 yields were obtained when cultures were supplied with air (20 cm3/min) and CO2 (5 cm3/min) via a sintered glass sparger.
机译:在发酵罐中研究了由金黄色葡萄球菌产生的毒性休克综合征毒素1(TSST-1)的动力学,在发酵罐中控制了曝气速率,大气成分,pH和温度。在指数期以最大速率合成了毒素。高细菌种群不一定伴随着高TSST-1产量。气生增加了TSST-1的产量,但过度通气有不利影响。通过增加毒素的产生速率和效率,添加二氧化碳可以提高TSST-1的产量。没有pH控制的培养物比保持在pH 5.5-7.5的培养物产生更多的TSST-1。当通过烧结的玻璃喷雾器向培养物提供空气(20 cm3 / min)和二氧化碳(5 cm3 / min)时,获得最大的TSST-1产量。

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