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首页> 外文期刊>Infection and immunity >Genomic Analysis of a Pathogenicity Island in Uropathogenic Escherichia coli CFT073: Distribution of Homologous Sequences among Isolates from Patients with Pyelonephritis, Cystitis, and CatheterAssociated Bacteriuria and from Fecal Samples
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Genomic Analysis of a Pathogenicity Island in Uropathogenic Escherichia coli CFT073: Distribution of Homologous Sequences among Isolates from Patients with Pyelonephritis, Cystitis, and CatheterAssociated Bacteriuria and from Fecal Samples

机译：致病性大肠杆菌CFT073中的致病岛的基因组分析：肾盂肾炎，膀胱炎和导管相关细菌尿患者以及粪便样本中分离株之间的同源序列分布

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Urinary tract infection is the most frequently diagnosed kidney and urologic disease and Escherichia coli is by far the most common etiologic agent. Uropathogenic strains have been shown to contain blocks of DNA termed pathogenicity islands (PAIs) which contribute to their virulence. We have defined one of these regions of DNA within the chromosome of a highly virulentE. coli strain, CFT073, isolated from the blood and urine of a woman with acute pyelonephritis. The 57,988-bp stretch of DNA has characteristics which define PAIs, including a size greater than 30 kb, the presence of insertion sequences, distinct segmentation of K-12 and J96 origin, GC content (42.9%) different from that of total genomic DNA (50.8%), and the presence of virulence genes (hly and pap). Within this region, we have identified 44 open reading frames; of these 44, 10 are homologous to entries in the complete K-12 genome sequence, 4 are nearly identical to the sequences of E. coli J96 encoding the HlyA hemolysin, 11 encode P fimbriae, and 19 show no homology to J96 or K-12 entries. To determine whether sequences found within the junctions of the PAI of CFT073 were common to other uropathogenic strains ofE. coli, 11 probes were isolated along the length of the PAI and were hybridized to dot blots of genomic DNA isolated from clinical isolates (67 from patients with acute pyelonephritis, 38 from patients with cystitis, 49 from patients with catheter-associated bacteriuria, and 27 from fecal samples). These sequences were found significantly more often in strains associated with the clinical syndromes of acute pyelonephritis (79%) and cystitis (82%) than in those associated with catheter-associated bacteriuria (58%) and in fecal strains (22%) (P < 0.001). From these regions, we have identified a putative iron transport system and genes other than hly and pap that may contribute to the virulent phenotype of uropathogenic E. coli strains.
机译：尿路感染是最常被诊断的肾脏和泌尿科疾病，而大肠杆菌是迄今为止最常见的病原体。业已证明，致病性菌株含有称为病原性岛（PAI）的DNA块，这些DNA有助于其致病性。我们已经在高毒性的 E染色体中定义了这些DNA区域之一。从一名患有急性肾盂肾炎的妇女的血液和尿液中分离出的大肠杆菌菌株CFT073。 57988 bp的DNA片段具有定义PAI的特征，包括大于30 kb的大小，插入序列的存在，K-12和J96来源的明显分割，GC含量（42.9％）与总基因组DNA的不同（50.8％），以及存在毒力基因（ hly 和 pap ）。在该区域内，我们已经确定了44个开放阅读框架。在这44个中，有10个与完整的K-12基因组序列中的条目同源，有4个与 E序列几乎相同。编码HlyA溶血素的大肠杆菌J96，编码P菌毛的11个和与J96或K-12条目没有同源性的J96。为了确定在CFT073的PAI的交界内发现的序列是否与其他EmE的尿路致病菌株相同。大肠杆菌，沿着PAI的长度分离了11种探针，并将其与从临床分离株中分离的基因组DNA进行斑点杂交（急性肾盂肾炎患者67例，膀胱炎患者38例，导管相关患者49例）细菌尿和粪便样本中的27个）。与急性肾盂肾炎和膀胱炎的临床综合征相关的菌株（79％）中发现这些序列的频率比与导管相关菌尿相关的菌株（58％）和粪便菌株（22％）中的发现频率更高em> P <0.001）。从这些区域，我们已经确定了可能的铁转运系统和除 hly 和 pap 以外的其他基因，这些基因可能有助于尿毒症性 E的强毒表型。大肠杆菌菌株。

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