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首页> 外文期刊>Infection and immunity >Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated
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Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated

机译：梅毒螺旋体和与病原有关的口服螺旋体的鞭毛蛋白（但不是鞭毛内鞘蛋白）被糖基化

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Glycosylation of the flagellar core proteins (FlaBs) was detected in Treponema pallidum Nichols and in the type or reference strains of seven oral Treponema species. In several nonmotile strains of oral treponemes, the FlaBs were undetectable by both antibody and glycan staining. In contrast, a spontaneous low-motility variant of T. vincenti￡i-related strain RitzA, OMZ 305A, lacked the flagellar sheath protein (FlaA) and the two glycan-staining FlaB bands of the wild type, but antibody labeling revealed a novel FlaB band with a lower relative molecular weight. A ca. 38-kDa component of isolated endoflagella of T. vincentii OMZ 800 was identified on Western blots as FlaA by monoclonal antibody (MAb) H9-2, which specifically labels the 37-kDa FlaA protein of T. pallidum. Glycan and H9-2 labeling patterns similar to those of T. pallidum were observed in whole-cell extracts of T. medium G7201 and of 10 strains classified as T. vincentii and as two T. vincentii-related taxons. These four groups were thus identified as cultivable pathogen (T. pallidum)-related oral spirochetes as defined by labeling with MAb H9-2. No H9-2 MAb-reactive component could be detected inT. amylovorum, T. denticola, T. maltophilum, T. pectinovorum, and the three subspecies of T. socranskii.
机译：在梅毒螺旋体和7种口服梅毒螺旋体的类型或参考菌株中检测到鞭毛核心蛋白（FlaBs）的糖基化。在几种口服分子的非运动系中，抗体和聚糖染色均无法检测到FlaB。相比之下， T的自发性低运动性变体。与芬奇霉素相关的菌株RitzA OMZ 305A缺乏鞭毛鞘蛋白（FlaA）和两个野生型的聚糖染色的FlaB带，但抗体标记显示了一个新的FlaB带，其较低相对分子量。大约通过单克隆抗体（MAb）H9-2在Western blots中鉴定出分离到的华氏锥虫OMZ 800内鞭毛的38 kDa成分为FlaA，该抗体特异性标记了 T的37 kDa FlaA蛋白。苍白的。聚糖和H9-2标记模式与 T相似。在 T的全细胞提取物中观察到了pallidum 。培养基 G7201和10个分类为 T的菌株。 vincentii 和两个 T。与vincentii 相关的分类单元。因此，这四个组被鉴定为可培养的病原体（ T。pallidum ）相关的口服螺旋体，通过用单克隆抗体H9-2进行标记来定义。在 T中未检测到H9-2 MAb反应性成分。淀粉菌素， T。 denticola ， T。麦芽糖， T。果胶菌和 T的三个亚种。 socranskii 。

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