• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Antigenic Analysis of Bordetella pertussis Filamentous Hemagglutinin with Phage Display Libraries and Rabbit Anti-Filamentous Hemagglutinin Polyclonal Antibodies
【24h】

Antigenic Analysis of Bordetella pertussis Filamentous Hemagglutinin with Phage Display Libraries and Rabbit Anti-Filamentous Hemagglutinin Polyclonal Antibodies

机译：噬菌体展示文库和兔抗丝状血凝素多克隆抗体对百日咳博德特氏菌丝状血凝素的抗原分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although substantial advancements have been made in the development of efficacious acellular vaccines against Bordetella pertussis, continued progress requires better understanding of the antigenic makeup of B. pertussis virulence factors, including filamentous hemagglutinin (FHA). To identify antigenic regions of FHA, phage display libraries constructed by using random fragments of the 10-kbp EcoRI fragment ofB. pertussis fhaB were affinity selected with rabbit anti-FHA polyclonal antibodies. Characterization of antibody-reactive clones displaying FHA-derived peptides identified 14 antigenic regions, each containing one or more epitopes. A number of clones mapped within regions containing known or putative FHA adhesin domains and may be relevant for the generation of protective antibodies. The immunogenic potential of the phage-displayed peptides was assessed indirectly by comparing their recognition by antibodies elicited by sodium dodecyl sulfate (SDS)-denatured and native FHA and by measuring the inhibition of this recognition by purified FHA. FHA residues 1929 to 2019 may contain the most dominant linear epitope of FHA. Clones mapping to this region accounted for ca. 20% of clones recovered from the initial library selection and screening procedures. They are strongly recognized by sera against both SDS-denatured and native FHA, and this recognition is readily inhibited by purified FHA. Given also that this region includes a factor X homolog (J. Sandros and E. Tuomanen, Trends Microbiol. 1:192–196, 1993) and that the single FHA epitope (residues 2001 to 2015) was unequivocally defined in a comparable study by E. Leininger et al. (J. Infect. Dis. 175:1423–1431, 1997), peptides derived from residues of 1929 to 2019 of FHA are strong candidates for future protection studies.
机译：尽管针对百日咳博德特氏菌的有效脱细胞疫苗的开发已取得实质性进展，但持续的进步需要更好地了解 B的抗原组成。百日咳的致病因子，包括丝状血凝素（FHA）。为了鉴定FHA的抗原区，噬菌体展示文库是通过使用 B的10-kbp Eco RI片段的随机片段构建的。百日咳fhaB 与兔抗FHA多克隆抗体进行亲和选择。显示FHA衍生肽的抗体反应性克隆的鉴定鉴定出14个抗原区，每个抗原区包含一个或多个表位。在含有已知或推定的FHA粘附素结构域的区域内定位的许多克隆，可能与保护性抗体的产生有关。噬菌体展示的肽的免疫原性潜力通过比较十二烷基硫酸钠（SDS）变性的FHA和天然FHA引起的抗体的识别性，以及通过测量纯化的FHA对这种识别的抑制性来间接评估。 1929年至2019年的FHA残基可能包含FHA最主要的线性表位。克隆到该区域的克隆约占。从最初的文库选择和筛选过程中回收了20％的克隆。血清对SDS变性和天然FHA均强烈识别它们，并且这种识别容易被纯化的FHA抑制。还应考虑到该区域包括X因子同源物（J. Sandros和E. Tuomanen，趋势微生物学，1：192-196，1993），并且在一项可比性研究中，FHA表位（2001至2015年的残基）明确定义。 E.Leininger等。 （J. Infect。Dis。175：1423–1431，1997），源自FHA 1929年至2019年残基的肽是未来保护研究的强有力候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号