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首页> 外文期刊>Infection and immunity >Structural and Genetic Analyses of O Polysaccharide from Actinobacillus actinomycetemcomitans Serotype f
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Structural and Genetic Analyses of O Polysaccharide from Actinobacillus actinomycetemcomitans Serotype f

机译：放线放线杆菌血清型f的O多糖的结构和遗传分析

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The oral bacterium Actinobacillus actinomycetemcomitans is implicated as a causative agent of localized juvenile periodontitis (LJP). A. actinomycetemcomitans is classified into five serotypes (a to e) corresponding to five structurally and antigenically distinct O polysaccharide (O-PS) components of their respective lipopolysaccharide molecules. Serotype b has been reported to be the dominant serotype isolated from LJP patients. We determined the lipopolysaccharide O-PS structure from A. actinomycetemcomitans CU1000, a strain isolated from a 13-year-old African-American female with LJP which had previously been classified as serotype b. The O-PS of strain CU1000 consisted of a trisaccharide repeating unit composed ofl-rhamnose and 2-acetamido-2-deoxy-d-galactose (molar ratio, 2:1) with the structure →2)-α-l-Rhap-(1–3)-2-O-(β-d-GalpNAc)-α-l-Rhap-(1→. O-PS from strain CU1000 was structurally and antigenically distinct from the O-PS molecules of the five known A. actinomycetemcomitans serotypes. Strain CU1000 was mutagenized with transposon IS903φkan, and three mutants that were deficient in O-PS synthesis were isolated. All three transposon insertions mapped to a single 1-kb region on the chromosome. The DNA sequence of a 13.1-kb region surrounding these transposon insertions contained a cluster of 14 open reading frames that was homologous to gene clusters responsible for the synthesis of A. actinomycetemcomitans serotype b, c, and e O-PS antigens. The CU1000 gene cluster contained two genes that were not present in serotype-specific O-PS antigen clusters of the other five knownA. actinomycetemcomitans serotypes. These data indicate that strain CU1000 should be assigned to a new A. actinomycetemcomitans serotype, designated serotype f. A PCR assay using serotype-specific PCR primers showed that 3 out of 20 LJP patients surveyed (15%) harbored A. actinomycetemcomitans strains carrying the serotype f gene cluster. The finding of an A. actinomycetemcomitansserotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted.
机译：口腔细菌放线放线杆菌（Actinobacillus actinomycetemcomitans）可能是引起局部少年牙周炎（LJP）的致病因子。 A。放线菌被分为五种血清型（a至e），分别与它们各自的脂多糖分子的五个结构和抗原性不同的O多糖（O-PS）成分相对应。据报道，血清型b是从LJP患者中分离的主要血清型。我们从 A确定了脂多糖O-PS结构。放线菌CU1000，是从一名13岁的具有LJP的非裔美国女性中分离出来的菌株，该菌株先前被分类为血清型b。菌株CU1000的O-PS由三糖重复单元组成，该三糖重复单元由1-鼠李糖和2-乙酰氨基-2-脱氧-d-半乳糖（摩尔比为2：1）组成，其结构为→2）-α-1-Rha< em> p -（1-3）-2- O -（β-d-Gal p NAc）-α-l-Rha p -（1→.. CU1000菌株的O-PS在结构和抗原上不同于五种已知的 A。放线菌科血清型的O-PS分子。CU1000菌株经转座子IS诱变分离出 903 φ kan 和三个O-PS合成缺陷的突变体，所有三个转座子插入均定位在染色体的单个1-kb区域。这些转座子插入周围的13.1kb区域的序列包含14个开放阅读框簇，这些阅读框与负责合成 A。放线菌血清型b，c和e O-PS的基因簇同源CU1000基因簇包含两个血清型特异性O-PS抗原c中不存在的基因其他五个已知的 A的光泽。放线菌的血清型。这些数据表明应将菌株CU1000分配给新的 A。放线菌的血清型，称为血清型f。使用血清型特异性PCR引物进行的PCR分析表明，在接受调查的20名LJP患者中，有3名（15％）患有 A。携带血清型f基因簇的放线放线杆菌菌株。 A的发现。放线菌的血清型与抗血清型b的抗血清具有血清学交叉反应性，这表明应该重新评估先前归类为血清型b的菌株。

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