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首页> 外文期刊>Infection and immunity >Nucleotide sequence of the variable domains within the major outer membrane protein gene from serovariants of Chlamydia trachomatis.
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Nucleotide sequence of the variable domains within the major outer membrane protein gene from serovariants of Chlamydia trachomatis.

机译：沙眼衣原体血清变异株主要外膜蛋白基因内可变域的核苷酸序列。

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During studies in which we serotyped large numbers of Chlamydia trachomatis clinical isolates by using monoclonal antibodies, three novel serological variants (D-, D*, and I-) were identified. To determine the molecular basis for the altered monoclonal antibody reactions of these strains and other previously identified variants (Da, Ia, and L2a), we determined the nucleotide sequences of the variable domains in their major outer membrane protein genes. Da, D-, and D* variants differed by a single nucleotide and also an amino acid in the carboxy terminus of variable domain IV (VDIV) from the D serovar. The L2a variant also differed from L2 by a single amino acid but in VDII. Ia variants differed in VDI, III, and IV and I- variants differed in all four VDs from the I serovar. These studies demonstrate the potential for using major outer membrane protein VD sequencing as a highly sensitive typing method and further identify immunologically reactive major outer membrane protein epitopes.
机译：在我们使用单克隆抗体对大量沙眼衣原体临床分离株进行血清分型的研究中，鉴定了三种新型血清学变体（D-，D *和I-）。为了确定这些菌株和其他先前鉴定的变体（Da，Ia和L2a）改变的单克隆抗体反应的分子基础，我们确定了其主要外膜蛋白基因中可变域的核苷酸序列。 Da，D-和D *变体的区别在于D血清型可变域IV（VDIV）的羧基末端中的单个核苷酸和氨基酸。 L2a变体与L2的区别还在于单个氨基酸，但在VDII中有所不同。 Ia变体在VDI，III和IV中有所不同，而I-变体在所有四个VD中均与I血清型不同。这些研究证明了使用主要外膜蛋白VD测序作为高灵敏度分型方法的潜力，并进一步鉴定了具有免疫反应性的主要外膜蛋白表位。

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