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首页> 外文期刊>Infection and immunity >Cellular internalization in the absence of invasin expression is promoted by the Yersinia pseudotuberculosis yadA product.
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Cellular internalization in the absence of invasin expression is promoted by the Yersinia pseudotuberculosis yadA product.

机译：不存在invasin表达的细胞内在化由假结核耶尔森氏菌yadA产物促进。

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The Yersinia pseudotuberculosis invasin protein is able to promote bacterial penetration into mammalian cells. Insertion mutations that eliminate production of this protein show residual internalization that is dependent on the presence of the Yersinia virulence plasmid. An enrichment procedure was used to isolate molecular clones containing regions of the virulence plasmid that confer this low-level uptake on Y. pseudotuberculosis inv mutants. All of the Y. pseudotuberculosis strains isolated from this procedure harbored plasmids containing a region encompassing the yadA gene, which encodes a previously identified adhesin associated with attachment to extracellular matrix proteins. All of the mutations isolated that affected internalization of one of the strains that survived the enrichment disrupted the yadA open reading frame. Furthermore, a strain that contained yadA sequences and no other region of the virulence plasmid was able to promote internalization of a Y. pseudotuberculosis inv mutant. Consistent with these results, an intact virulence plasmid containing an insertion mutation in yadA was as defective as a plasmid-cured strain at promoting uptake of Y. pseudotuberculosis inv mutants. These results indicate that the product of the yadA gene is responsible for the plasmid-dependent entry observed in Y. pseudotuberculosis inv mutants.
机译：耶尔森氏假结核耶尔森菌素蛋白能够促进细菌渗透进入哺乳动物细胞。消除该蛋白产生的插入突变显示出残余内在化，其取决于耶尔森氏菌毒力质粒的存在。使用富集程序来分离分子克隆，该分子克隆包含赋予假单胞菌inv突变体这种低水平摄取的毒力质粒区域。从该程序中分离出的所有假结核耶尔森氏菌菌株都含有含有包含yadA基因区域的质粒，该区域编码先前鉴定的与细胞外基质蛋白附着相关的粘附素。分离出的所有突变均影响了在富集中幸存下来的菌株之一的内部化，从而破坏了yadA开放阅读框。此外，含有yadA序列且无毒力质粒其他区域的菌株能够促进假结核耶尔森氏菌inv突变体的内在化。与这些结果一致，在yadA中含有插入突变的完整毒力质粒与质粒固化菌株在促进对假结核耶尔森氏菌inv突变体的摄取方面一样具有缺陷。这些结果表明，yadA基因的产物负责在假结核耶尔森氏菌inv突变体中观察到的质粒依赖性进入。

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