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首页> 外文期刊>Infection and immunity >Nucleotide sequence of the Streptococcus gordonii PK488 coaggregation adhesin gene, scaA, and ATP-binding cassette.
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Nucleotide sequence of the Streptococcus gordonii PK488 coaggregation adhesin gene, scaA, and ATP-binding cassette.

机译：戈登链球菌PK488共聚集粘附素基因，scaA和ATP结合盒的核苷酸序列。

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Human oral viridans group streptococci that coaggregate with Actinomyces naeslundii PK606 express surface proteins related to ScaA, the coaggregation-mediating adhesin of Streptococcus gordonii PK488 (R. N. Andersen, N. Ganeshkumar, and P. E. Kolenbrander, Infect. Immun. 61:981-987, 1993). The nucleotide sequence of the 6,125-bp EcoRI insert of pRA1, containing scaA, the gene encoding ScaA, was determined. Six open reading frames (ORFs) were identified. The orientation of four ORFs, two upstream (ORF 1 and ORF 2) and one downstream (ORF 4) of scaA (ORF 3), indicated transcription in one direction, whereas ORF 5 and ORF 6 were transcribed divergently. Computer analysis of the deduced amino acid sequences identified a consensus binding site for ATP (GxxGxGKS) in the putative 28,054-Da protein encoded by ORF 1. ORF 2 potentially encoded a hydrophobic protein of 29,705 Da with six potential membrane-spanning regions. ScaA was 310 amino acids, 34,787 Da, and contained the lipoprotein consensus sequence LxxC, also reported for the ScaA-related proteins SsaB, FimA, and PsaA from Streptococcus sanguis 12, Streptococcus parasanguis FW213, and Streptococcus pneumoniae R36A, respectively. ORF 4 potentially encoded a 163-amino-acid protein of 17,912 Da, which was nearly identical to the downstream adjacent gene products of ssaB, fimA, and psaA. No significant homology with other proteins was found with the putative ORF 5 gene product, a 229-amino-acid protein of 25,107 Da. ORF 6 was incomplete and encoded a protein larger than 564 amino acids. This putative protein had a consensus Zn2+ binding motif, HExxH, found among bacterial thermolysins and mammalian neutral endopeptidases and was 40% identical to a homologous 210-amino-acid region of human enkephalinase. The genetic organization of ORFs 1, 2, and 3 was similar to those of the bacterial periplasmic-binding protein-dependent transport systems of gram-negative bacteria and binding-lipoprotein-dependent transport systems of gram-positive bacteria, and these genes appeared to encode ABC (ATP-binding cassette) proteins. This report describes a cell-to-cell adherence function associated with an ATP-binding cassette.
机译：与内脏放线菌PK606共聚集的人类口腔翠绿菌群链球菌表达与ScaA有关的表面蛋白，该蛋白是戈登链球菌PK488的共聚集介导粘附素（RN Andersen，N.Ganeshkumar和PE Kolenbrander，Infect.Immun.61：981-987，1993 ）。确定了包含scaA（编码ScaA的基因）的pRA1的6,125 bp EcoRI插入片段的核苷酸序列。确定了六个开放阅读框（ORF）。 scaA（ORF 3）的四个ORF（两个上游（ORF 1和ORF 2）和一个下游（ORF 4））的方向指示一个方向的转录，而ORF 5和ORF 6的转录是不同的。推导的氨基酸序列的计算机分析确定了由ORF 1编码的推定的28,054-Da蛋白中ATP的共有结合位点（GxxGxGKS）。ORF2可能编码29,705 Da的疏水蛋白，具有六个潜在的跨膜区域。 ScaA是310个氨基酸，34,787 Da，并包含脂蛋白共有序列LxxC，也报道了来自血链球菌12，副产链球菌FW213和肺炎链球菌R36A的ScaA相关蛋白SsaB，FimA和PsaA。 ORF 4可能编码一个17,912 Da的163个氨基酸的蛋白质，与ssaB，fimA和psaA的下游相邻基因产物几乎相同。推定的ORF 5基因产物（一个25,107 Da的229个氨基酸的蛋白质）未发现与其他蛋白质的显着同源性。 ORF 6不完整，编码的蛋白质大于564个氨基酸。该推定蛋白在细菌嗜热菌蛋白酶和哺乳动物中性内肽酶中发现了共有的Zn2 +结合基序HExxH，与人脑啡肽酶的210个氨基酸同源区域具有40％的同一性。 ORF 1、2和3的遗传组织类似于革兰氏阴性细菌的细菌周质结合蛋白依赖性转运系统和革兰氏阳性细菌的结合脂蛋白依赖性转运系统，这些基因似乎编码ABC（ATP结合盒）蛋白。该报告描述了与ATP结合盒相关的细胞间粘附功能。

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